ArticlePloS one2012
Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 61 citations in OpenAlex.
- Deciphering the role of MFGE8 in lactation using CRISPR-CAS9 based gene editing in Buffalo mammary epithelial cells.Scientific reports · 2025Article
- Novel Interacting Partners of MGP-40, a Chitinase-Like Protein in Buffalo Mammary Epithelial Cells.Cell biochemistry and biophysics · 2025Article
- Cellular characteristics and milk component productivity of primary bovine mammary cells for cell-cultured milk component production.Journal of animal science and technology · 2025Article
- Current status and challenges for cell-cultured milk technology: a systematic review.Journal of animal science and biotechnology · 2024Review
- Successful editing and maintenance of lactogenic gene expression in primary bovine mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2023Article
- Novel Insight into the Role of Squalene Epoxidase (International journal of molecular sciences · 2023Article
- Review
- Article
- Tissue-Specific Expression of Circ_015343 and Its Inhibitory Effect on Mammary Epithelial Cells in Sheep.Frontiers in veterinary science · 2022Article
- Article
- In-depth proteome analysis of more than 12,500 proteins in buffalo mammary epithelial cell line identifies protein signatures for active proliferation and lactation.Scientific reports · 2020Article
- New insights into the catalytic inactivity of mammary gland protein-40, a chitinase-like protein expressed during mammary gland involution.Molecular biology reports · 2019Article
- Development and validation of immortalized bovine mammary epithelial cell line as an in vitro model for the study of mammary gland functions.Cytotechnology · 2018Article
- Recombinant purified buffalo leukemia inhibitory factor plays an inhibitory role in cell growth.PloS one · 2018Article
- Examination of the xanthosine response on gene expression of mammary epithelial cells using RNA-seq technology.Journal of animal science and technology · 2018Article
- Impact of Heat Stress on Cellular and Transcriptional Adaptation of Mammary Epithelial Cells in Riverine Buffalo (Bubalus Bubalis).PloS one · 2016Article
- Thyroid hormone responsive protein spot 14 enhances lipogenesis in bovine mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2015Article
- Article
- Lineage-specific markers of goat mammary cells in primary culture.In vitro cellular & developmental biology. Animal · 2014Article
- Bovine mammary epithelial cell cultures for the study of mammary gland functions.In vitro cellular & developmental biology. Animal · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe objective of this study was to establish the buffalo mammary epithelial cell line (BuMEC) and characterize its mammary specific functions. METHODOLOGY: Buffalo mammary tissue collected from the slaughter house was processed enzymatically to obtain a heterogenous population of cells containing both epithelial and fibroblasts cells. Epithelial cells were purified by selective trypsinization and were grown in a plastic substratum. The purified mammary epithelial cells (MECs) after several passages were characterized for mammary specific functions by immunocytochemistry, RT-PCR and western blot. PRINCIPAL
findingsThe established buffalo mammary epithelial cell line (BuMEC) exhibited epithelial cell characteristics by immunostaining positively with cytokeratin 18 and negatively with vimentin. The BuMEC maintained the characteristics of its functional differentiation by expression of β-casein, κ-casein, butyrophilin and lactoferrin. BuMEC had normal growth properties and maintained diploid chromosome number (2n = 50) before and after cryopreservation. A spontaneously immortalized buffalo mammary epithelial cell line was established after 20 passages and was continuously subcultured for more than 60 passages without senescence.
conclusionsWe have established a buffalo mammary epithelial cell line that can be used as a model system for studying mammary gland functions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.