ArticlePloS one2012
Membrane fusion proteins of type I secretion system and tripartite efflux pumps share a binding motif for TolC in gram-negative bacteria.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 51 citations in OpenAlex.
- TolC Is Required for a Mixed-Linkage β-Glucan (MLG) Biosynthesis: Engineering Bacteria for MLG Overproduction.Microbial biotechnology · 2026Article
- Review
- A coevolution experiment betweenMicrobiology (Reading, England) · 2023Article
- Review
- Stress response and virulence factors in bacterial pathogens relevant for Chilean aquaculture: current status and outlook of our knowledge.Biological research · 2022Review
- Review
- Tenacibaculosis caused byFrontiers in cellular and infection microbiology · 2022Review
- Identification of the Key Enzymes in WL Gum Biosynthesis and Critical Composition in Viscosity Control.Frontiers in bioengineering and biotechnology · 2022Article
- Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.Chemical reviews · 2021Review
- Confronting Tigecycline-ResistantFrontiers in microbiology · 2020Article
- Identification of binding residues between periplasmic adapter protein (PAP) and RND efflux pumps explains PAP-pump promiscuity and roles in antimicrobial resistance.PLoS pathogens · 2019Article
- Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system.Journal of microbiology (Seoul, Korea) · 2019Review
- ATP-Binding Cassette Transporter VcaM from Vibrio cholerae is Dependent on the Outer Membrane Factor Family for Its Function.International journal of molecular sciences · 2018Article
- Article
- The Complete Genome Sequence of the Fish PathogenFrontiers in microbiology · 2017Article
- Type I Protein Secretion-Deceptively Simple yet with a Wide Range of Mechanistic Variability across the Family.EcoSal Plus · 2016Review
- Bacterial Secretion Systems: An Overview.Microbiology spectrum · 2016Review
- Intestinal M cells.Journal of biochemistry · 2016Review
- Molecular architecture of the bacterial tripartite multidrug efflux pump focusing on the adaptor bridging model.Journal of microbiology (Seoul, Korea) · 2015Review
- Functional analysis of Vibrio vulnificus RND efflux pumps homologous to Vibrio cholerae VexAB and VexCD, and to Escherichia coli AcrAB.Journal of microbiology (Seoul, Korea) · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Hly translocator complex of Escherichia coli catalyzes type I secretion of the toxin hemolysin A (HlyA). In this complex, HlyB is an inner membrane ABC (ATP Binding Cassette)-type transporter, TolC is an outer membrane channel protein, and HlyD is a periplasmic adaptor anchored in the inner membrane that bridges HlyB to TolC. This tripartite organization is reminiscent of that of drug efflux systems such as AcrA-AcrB-TolC and MacA-MacB-TolC of E. coli. We have previously shown the crucial role of conserved residues located at the hairpin tip region of AcrA and MacA adaptors during assembly of their cognate systems. In this study, we investigated the role of the putative tip region of HlyD using HlyD mutants with single amino acid substitutions at the conserved positions. In vivo and in vitro data show that all mutations abolished HlyD binding to TolC and resulted in the absence of HlyA secretion. Together, our results suggest that, similarly to AcrA and MacA, HlyD interacts with TolC in a tip-to-tip manner. A general model in which these conserved interactions induce opening of TolC during drug efflux and type I secretion is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.