ArticlePloS one2012
Screening of 71 P. multocida proteins for protective efficacy in a fowl cholera infection model and characterization of the protective antigen PlpE.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Design of a Multi-Epitope Vaccine Against OvineMicroorganisms · 2026Article
- Comparative analysis of shared and unique mechanisms important for diverse strains of Pasteurella multocida to cause systemic infection in mice.PLoS pathogens · 2025Article
- Immunoprotective effects of extracellular products ofFrontiers in microbiology · 2025Article
- Evaluation of a multi-epitope vaccine PME forFrontiers in immunology · 2025Article
- Genomic characterization and comparative genomic analysis of HS-associated Pasteurella multocida serotype B:2 strains from Pakistan.BMC genomics · 2023Article
- Recombinant-attenuated Salmonella enterica serovar Choleraesuis vector expressing the PlpE protein of Pasteurella multocida protects mice from lethal challenge.BMC veterinary research · 2023Article
- Combinations but Not a Single PlpE Epitope Induces Host Protective Immunity against Pasteurella multocida.Infection and immunity · 2023Article
- Reverse vaccinology-based identification of a novel surface lipoprotein that is an effective vaccine antigen against bovine infections caused by Pasteurella multocida.PLoS pathogens · 2023Article
- Immunogenicity and protective efficacy of the recombinantFrontiers in immunology · 2022Article
- Novel multi-strain probiotics reduces Pasteurella multocida induced fowl cholera mortality in broilers.Scientific reports · 2021Article
- In silico Analysis of Pasteurella multocida PlpE Protein Epitopes As Novel Subunit Vaccine Candidates.Iranian biomedical journal · 2021Article
- PlpE Epitopes ofAdvanced biomedical research · 2020Article
- Identification of Novel Acinetobacter baumannii Type VI Secretion System Antibacterial Effector and Immunity Pairs.Infection and immunity · 2018Article
- OmpA protein sequence-based typing and virulence-associated gene profiles of Pasteurella multocida isolates associated with bovine haemorrhagic septicaemia and porcine pneumonic pasteurellosis in Thailand.BMC veterinary research · 2017Article
- Recombinant proteins from Gallibacterium anatis induces partial protection against heterologous challenge in egg-laying hens.Veterinary research · 2016Article
- Immune efficacy of five novel recombinant Bordetella bronchiseptica proteins.BMC veterinary research · 2015Article
- Pasteurella multocida: from zoonosis to cellular microbiology.Clinical microbiology reviews · 2013Review
- Comparative genome analysis of an avirulent and two virulent strains of avian Pasteurella multocida reveals candidate genes involved in fitness and pathogenicity.BMC microbiology · 2013Article
- Occurrence ofIrish veterinary journalArticle
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThere is a strong need for a recombinant subunit vaccine against fowl cholera. We used a reverse vaccinology approach to identify putative secreted or cell surface associated P. multocida proteins that may represent potential vaccine candidate antigens. PRINCIPAL
findingsA high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteins for vaccine trials. Of the 71 proteins tested, only one, PlpE in denatured insoluble form, protected chickens against fowl cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To explore the role of PlpE during infection and immunity, a plpE mutant was generated. The plpE mutant strain retained full virulence for mice.
conclusionThese studies show that PlpE is a surface exposed protein and was the only protein of 71 tested that was able to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denatured recombinant protein stimulating protection against fowl cholera.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.