ArticleHead & neck2013
Orosphere assay: a method for propagation of head and neck cancer stem cells.
Article in Head & neck, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effects of extracellular acidity on resistance to chemotherapy treatment: a systematic review.Medical oncology (Northwood, London, England) · 2018Pooled it
- Comparative evaluation of anticancer potential of Spilanthes paniculata leaf and flower essential oil using annexin V and orosphere formation in oral cancer cell line.Medical oncology (Northwood, London, England) · 2024Article
- Cancer Stem Cells in Oral Squamous Cell Carcinoma: A Narrative Review on Experimental Characteristics and Methodological Challenges.Biomedicines · 2024Review
- A Kinome-Wide Synthetic Lethal CRISPR/Cas9 Screen Reveals That mTOR Inhibition Prevents Adaptive Resistance to CDK4/CDK6 Blockade in HNSCC.Cancer research communications · 2024Article
- Review
- Three-dimensional (3D) cell culture: a valuable step in advancing treatments for human hepatocellular carcinoma.Cancer cell international · 2022Review
- Graphene-Based Materials for Efficient Neurogenesis.Advances in experimental medicine and biology · 2022Article
- The IL-6R and Bmi-1 axis controls self-renewal and chemoresistance of head and neck cancer stem cells.Cell death & disease · 2021Article
- SOD2 Enhancement by Long-Term Inhibition of the PI3K Pathway Confers Multi-Drug Resistance and Enhanced Tumor-Initiating Features in Head and Neck Cancer.International journal of molecular sciences · 2021Article
- Propolis reduces the stemness of head and neck squamous cell carcinoma.Archives of oral biology · 2021Article
- Organoids in Tissue Transplantation.Advances in experimental medicine and biology · 2021Review
- Molecular and Cellular Modelling of Salivary Gland Tumors Open New Landscapes in Diagnosis and Treatment.Cancers · 2020Review
- SCF/C-Kit Signaling Induces Self-Renewal of Dental Pulp Stem Cells.Journal of endodontics · 2020Article
- Endothelial-Initiated Crosstalk Regulates Dental Pulp Stem Cell Self-Renewal.Journal of dental research · 2020Article
- Radiotherapy targeting cancer stem cells "awakens" them to induce tumour relapse and metastasis in oral cancer.International journal of oral science · 2020Review
- Cancer Stem Cells in Head and Neck Squamous Cell Carcinoma: Identification, Characterization and Clinical Implications.Cancers · 2019Review
- Head and neck cancer organoids established by modification of the CTOS method can be used to predict in vivo drug sensitivity.Oral oncology · 2018Article
- 2D and 3D cell cultures - a comparison of different types of cancer cell cultures.Archives of medical science : AMS · 2018Article
- FGFR signaling regulates resistance of head and neck cancer stem cells to cisplatin.Oncotarget · 2018Article
- Targeting histone deacetylase and NFκB signaling as a novel therapy for Mucoepidermoid Carcinomas.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
backgroundRecent evidence suggests that head and neck squamous cell carcinomas (HNSCCs) harbor a small subpopulation of highly tumorigenic cells, designated cancer stem cells. A limiting factor in cancer stem cell research is the intrinsic difficulty of expanding cells in an undifferentiated state in vitro.
methodsHere, we describe the development of the orosphere assay, a method for the study of putative head and neck cancer stem cells. An orosphere is defined as a nonadherent colony of cells sorted from primary HNSCCs or from HNSCC cell lines and cultured in 3-dimensional soft agar or ultralow attachment plates. Aldehyde dehydrogenase activity and CD44 expression were used here as stem cell markers.
resultsThis assay allowed for the propagation of head and neck cancer cells that retained stemness and self-renewal.
conclusionThe orosphere assay is well suited for studies designed to understand the pathobiology of head and neck cancer stem cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.