ArticleTransgenic research2013
Expression of a rice chitinase gene in transgenic banana ('Gros Michel', AAA genome group) confers resistance to black leaf streak disease.
Article in Transgenic research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 110 citations in OpenAlex.
- Genome-Wide Analysis Reveals Chitinases as Putative Defense-Related Proteins Against Fungi in the Genomes ofPlants (Basel, Switzerland) · 2025Article
- Shaping the future of bananas: advancing genetic trait regulation and breeding in the postgenomics era.Horticulture research · 2025Article
- Histological and transcriptome analysis uncover a robust early PTI and ETI-associated immune response inFrontiers in plant science · 2025Article
- Systematic analysis and functional characterization of the chitinase gene family in Fagopyrum tataricum under salt stress.BMC plant biology · 2024Article
- Precision genetics tools for genetic improvement of banana.The plant genome · 2024Review
- Co-overexpression of chitinase and β-1,3-glucanase significantly enhanced the resistance of Iranian wheat cultivars to Fusarium.BMC biotechnology · 2024Article
- Characterization of soybean chitinase genes induced by rhizobacteria involved in the defense againstFrontiers in plant science · 2024Article
- Transcriptome Profiling of the Resistance Response ofInternational journal of molecular sciences · 2022Article
- Review
- Genome-Wide Identification and Expression Analysis ofPlants (Basel, Switzerland) · 2022Article
- Genetic Characteristics and Metabolic Interactions betweenPlants (Basel, Switzerland) · 2022Review
- Perspective for genomic-enabled prediction against black sigatoka disease and drought stress in polyploid species.Frontiers in plant science · 2022Review
- Article
- Melatonin Mitigates the Infection ofAntioxidants (Basel, Switzerland) · 2020Article
- Knockdown of the chitin-binding protein family gene CaChiIV1 increased sensitivity to Phytophthora capsici and drought stress in pepper plants.Molecular genetics and genomics : MGG · 2019Article
- Cucumber mosaic virus-induced gene silencing in banana.Scientific reports · 2019Article
- RNAi-mediated protection against banana diseases and pests.3 Biotech · 2019Review
- Classification and Genome-Wide Analysis of Chitin-Binding Proteins Gene Family in Pepper (Capsicum annuum L.) and Transcriptional Regulation to Phytophthora capsici, Abiotic Stresses and Hormonal Applications.International journal of molecular sciences · 2018Article
- Genetic Engineering: A Possible Strategy for Protein-Energy Malnutrition Regulation.Molecular biotechnology · 2017Review
- Comparative Study of Lectin Domains in Model Species: New Insights into Evolutionary Dynamics.International journal of molecular sciences · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic banana (Musa acuminata 'Gros Michel') integrating either of two rice chitinase genes was generated and its resistance to Black Leaf Streak disease caused by the fungus Mycosphaerella fijiensis was tested using a leaf disk bioassay. PCR screening indicated the presence of the hpt selectable marker gene in more than 90 % of the lines tested, whereas more than three quarters of the lines contained the linked rice chitinase gene resulting in a co-transformation frequency of at least 71.4 %. Further, a unique stable integration of the transgenes in each line revealed some false negative PCR results and the expected co-transformation frequency of 100 %. The transgene insert number per line ranged from 1 to 5 and single transgene insert lines (25 % of all) were identified. Considerable delay in disease development (up to 63 days post-incoculation) over a monitoring period of 108 days occurred in nine lines with extracellularly targeted chitinase out of 17 transgenic lines tested and their necrotic leaf area decreased by 73-94 % compared to the untransformed susceptible control line. Finally, correlation between symptom development and rice chitinase expression was confirmed in two lines by Western analysis. The potential of rice chitinase genes to enhance resistance against M. fijiensis in banana was demonstrated as well as the usefulness of the leaf disk bioassay for early disease screening in transgenic banana lines.
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