ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2012
Spectinomycin resistance mutations in the rrn16 gene are new plastid markers in Medicago sativa.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 13 citations in OpenAlex.
- Analysis of the variation and genetic stability of chloroplast genome of Pinus taeda.BMC genomics · 2026Article
- Glycyrrhiza plastid paternal inheritance and a new DNA barcode provide new strategies for molecular identification of three medicinal licorice hybrid complexes.BMC plant biology · 2025Article
- Protocol: a simple method for biosensor visualization of bacterial quorum sensing and quorum quenching interaction on Medicago roots.Plant methods · 2022Article
- Inhibition of chloroplast translation as a new target for herbicides.RSC chemical biology · 2022Article
- Natural variation in sensitivity to a loss of chloroplast translation in Arabidopsis.Plant physiology · 2014Article
- Two distinct plastid genome configurations and unprecedented intraspecies length variation in the accD coding region in Medicago truncatula.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
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Abstract
We report here the isolation of spectinomycin-resistant mutants in cultured cells of Medicago sativa line RegenSY-T2. Spectinomycin induces bleaching of cultured alfalfa cells due to inhibition of protein synthesis on the prokaryotic type 70S plastid ribosomes. Spontaneous mutants resistant to spectinomycin bleaching were identified by their ability to form green shoots on plant regeneration medium containing selective spectinomycin concentrations in the range of 25-50 mg/l. Sequencing of the plastid rrn16 gene revealed that spectinomycin resistance is due to mutations in a conserved stem structure of the 16S rRNA. Resistant plants transferred to the greenhouse developed normally and produced spectinomycin-resistant seed progeny. In light of their absence in soybean, a related leguminous plant, the isolation of spectinomycin-resistant mutants in M. sativa was unexpected. The new mutations are useful for the study of plastid inheritance, as demonstrated by detection of predominantly paternal plastid inheritance in the RegenSY-T2 × Szapko57 cross, and can be used as selective markers in plastid transformation vectors to obtain cisgenic plants.
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