Trial reportBlood transfusion = Trasfusione del sangue2013
ABO chimerism determined by real-time polymerase chain reaction analysis after ABO-incompatible haematopoietic stem cell transplantation.
Trial report in Blood transfusion = Trasfusione del sangue, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed, 8 citations in OpenAlex.
- Review
- Post-hematopoietic stem cell transplantation immune-mediated anemia: a literature review and novel therapeutics.Blood advances · 2022Review
- Rapid ABO genotyping by high-speed droplet allele-specific PCR using crude samples.Journal of clinical laboratory analysis · 2018Article
- ABO chimerism with a minor allele detected by the peptide nucleic acid-mediated polymerase chain reaction clamping method.Blood transfusion = Trasfusione del sangue · 2014Article
- ABO genotyping: the quest for clinical applications.Blood transfusion = Trasfusione del sangue · 2013Article
Corrections and comments
- Commented on by
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe dynamic alteration of ABO blood group in ABO-incompatible haematopoietic stem cell transplantation can be well defined by ABO chimerism analysis. In view of the intrinsic difference in ABO phenotypes or genotypes between donor and recipient in ABO incompatible transplantation, ABO allele-associated nucleotide polymorphic sites could theoretically be used as available informative markers for chimerism analysis. MATERIALS AND
methodsWe chose nucleotide polymorphism sites (261, 467, 802, 803 and 1,061) of common ABO alleles to use as markers from 76 randomly chosen donors and assessed the limit of linear detection of a specifically designed real-time quantitative polymerase chain reaction using SYBR Green I dye with these sites for a chimerism assay.
resultsWe successfully established the real-time quantitative polymerase chain reaction for detecting 261, 467(mutated) and 803 sites and their limits of linear detection, which were at least 10(-3), 10(-2) and 10(-2), respectively. The limits of linear detection between heterozygous DNA and homozygous DNA with 261 or 803 sites were not significantly different. DISCUSSION: ABO chimerism can be well analysed by real-time polymerase chain reaction with ABO gene-associated nucleotide polymorphic sites. This strategy could be very beneficial for the early and accurate judgement of the crucial point of transition in order to plan a safe transfusion strategy following ABO-incompatible transplantation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.