ArticleNature protocols2012
Optogenetic manipulation of neural circuits and behavior in Drosophila larvae.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- A pair of dopaminergic neurons DAN-c1 mediateeLife · 2025Article
- Nociception inCold Spring Harbor protocols · 2025Article
- Optogenetic Stimulation of Nociceptive Escape Behaviors inCold Spring Harbor protocols · 2025Article
- Optogenetic control ofJournal of microbiology & biology education · 2024Article
- Modeling exercise using optogenetically contractible Drosophila larvae.BMC genomics · 2022Article
- Optogenetic and thermogenetic manipulation of defined neural circuits and behaviors inLearning & memory (Cold Spring Harbor, N.Y.) · 2022Article
- Review
- Neural Design Principles for Subjective Experience: Implications for Insects.Frontiers in behavioral neuroscience · 2021Article
- Article
- Optogenetics inspired transition metal dichalcogenide neuristors for in-memory deep recurrent neural networks.Nature communications · 2020Article
- Frameshift mutations ofDisease models & mechanisms · 2020Article
- Calcium-Activated Calpain Specifically Cleaves Glutamate Receptor IIA But Not IIB at theThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- BMP signaling downstream of the Highwire E3 ligase sensitizes nociceptors.PLoS genetics · 2018Article
- FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation.Journal of visualized experiments : JoVE · 2018Article
- Functional Imaging and Optogenetics inGenetics · 2018Review
- A sleep state ineLife · 2018Article
- Nanotechnology for Neuroscience: Promising Approaches for Diagnostics, Therapeutics and Brain Activity Mapping.Advanced functional materials · 2017Article
- Serotonergic Modulation Enables Pathway-Specific Plasticity in a Developing Sensory Circuit in Drosophila.Neuron · 2017Article
- Sensory integration and neuromodulatory feedback facilitate Drosophila mechanonociceptive behavior.Nature neuroscience · 2017Article
- Neuron class-specific requirements for Fragile X Mental Retardation Protein in critical period development of calcium signaling in learning and memory circuitry.Neurobiology of disease · 2016Article
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3 authors.
Funding
Abstract
Optogenetics is a powerful tool that enables the spatiotemporal control of neuronal activity and circuits in behaving animals. Here, we describe our protocol for optical activation of neurons in Drosophila larvae. As an example, we discuss the use of optogenetics to activate larval nociceptors and nociception behaviors in the third-larval instar. We have previously shown that, using spatially defined GAL4 drivers and potent UAS (upstream activation sequence)-channelrhodopsin-2∷YFP transgenic strains developed in our laboratory, it is possible to manipulate neuronal populations in response to illumination by blue light and to test whether the activation of defined neural circuits is sufficient to shape behaviors of interest. Although we have only used the protocol described here in larval stages, the procedure can be adapted to study neurons in adult flies--with the caveat that blue light may not sufficiently penetrate the adult cuticle to stimulate neurons deep in the brain. This procedure takes 1 week to culture optogenetic flies and ~1 h per group for the behavioral assays.
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