ArticleInvestigative ophthalmology & visual science2012
The use of fluorescent quenching in studying the contribution of evaporation to tear thinning.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 76 citations in OpenAlex.
- Tear Film Imager Findings Support a New Perspective on the Mechanisms of Tear Film Thinning in Healthy Subjects.Investigative ophthalmology & visual science · 2026Article
- Tear Clearance Rate Assessment with a Modified Slit Lamp Biomicroscope.Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists) · 2026Article
- A Droplet Evaporimeter for Evaluating Eye Care Formulations.Translational vision science & technology · 2026Article
- Localized Evaporative Cooling Explains Observed Ocular Surface-Temperature Patterns.Investigative ophthalmology & visual science · 2024Article
- Ferroptosis in the Lacrimal Gland Is Involved in Dry Eye Syndrome Induced by Corneal Nerve Severing.Investigative ophthalmology & visual science · 2023Article
- Characterization of the thickness of the Tear Film Lipid Layer in Meibomian Gland Dysfunction using high resolution optical microscopy.The ocular surface · 2022Article
- A Cu(II) Indicator Platform Based on Cu(II) Induced Swelling that Changes the Extent of Fluorescein Self-Quenching.Polymers · 2019Article
- Changes in Corneal Detection Thresholds After Repeated Tear Film Instability.Investigative ophthalmology & visual science · 2019Article
- Dynamics of Fluorescent Imaging for Rapid Tear Thinning.Bulletin of mathematical biology · 2019Article
- On tear film breakup (TBU): dynamics and imaging.Mathematical medicine and biology : a journal of the IMA · 2018Article
- Mechanisms, imaging and structure of tear film breakup.The ocular surface · 2018Review
- Fluorescence lifetime imaging microscopy reveals quenching of fluorescein within corneal epithelium.Experimental eye research · 2016Article
- Computed tear film and osmolarity dynamics on an eye-shaped domain.Mathematical medicine and biology : a journal of the IMA · 2016Article
- Static and Dynamic Measurement of Ocular Surface Temperature in Dry Eyes.Journal of ophthalmology · 2016Article
- The Effects of Increasing Ocular Surface Stimulation on Blinking and Tear Secretion.Investigative ophthalmology & visual science · 2015Article
- Dynamics and function of the tear film in relation to the blink cycle.Progress in retinal and eye research · 2015Review
- Glutathione-coordinated [2Fe-2S] cluster: a viable physiological substrate for mitochondrial ABCB7 transport.Chemical communications (Cambridge, England) · 2015Article
- Direct observation and validation of fluorescein tear film break-up patterns by using a dual thermal-fluorescent imaging system.Biomedical optics express · 2014Article
- Tear film dynamics with evaporation, wetting, and time-dependent flux boundary condition on an eye-shaped domain.Physics of fluids (Woodbury, N.Y. : 1994) · 2014Article
- A model for tear film thinning with osmolarity and fluorescein.Investigative ophthalmology & visual science · 2014Article
Corrections and comments
- Commented on by
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
purposeThe purpose of our study was to test the prediction that if the tear film thins due to evaporation, rather than tangential flow, a high concentration of fluorescein in the tear film would show a greater reduction in fluorescent intensity compared to a low concentration of fluorescein due to self-quenching at high concentrations.
methodsTear film thickness, thinning rate, and fluorescent intensity were measured continuously and simultaneously with a modified spectral interferometer in 30 healthy subjects with two different concentrations (2% followed by 10%) of 1 μL of liquid fluorescein on the eye. Measurements of fluorescein self-quenching (fluorescent efficiency as a function of fluorescein concentration) are described in an Appendix and are reported in arbitrary units.
resultsUnder low and high fluorescein concentration conditions, there were no differences in tear film thickness (P = 0.09) or thinning rates (P = 0.76). While the mean initial fluorescent intensity was similar between groups (637.47 ± 381.47 vs. 672.09 ± 649.72, P = 0.55), the mean rate of fluorescent decay was 4-fold faster in the high (16.57 ± 29.34) than in the low (4.11 ± 6.78) concentration group (P < 0.01).
conclusionsThe large difference in the rate of fluorescent decay between groups can be explained by the effects of evaporation and self quenching of fluorescein; the latter is expected to be greater for high than for low fluorescein concentration. Fluorescence decay due to tangential flow would be expected to be similar at high and low fluorescein concentrations. This supports previous evidence that evaporation has the primary role in normal tear thinning between blinks.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.