ArticlePlant methods2012
Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry.
Article in Plant methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- Interactomics-driven discovery of alkaloid biosynthetic pathways in kratom.bioRxiv : the preprint server for biology · 2026Article
- A Live Cell Protein Complementation Assay for ORFeome-Wide Probing of Human HOX Interactomes.Cells · 2023Article
- Protein-protein interactions in plant antioxidant defense.Frontiers in plant science · 2022Review
- A bimolecular fluorescence complementation flow cytometry screen for membrane protein interactions.Scientific reports · 2021Article
- Genetically Encoded Sensor Cells for the Screening of Glucocorticoid Receptor (GR) Effectors in Herbal Extracts.Biosensors · 2021Article
- Article
- Arabidopsis Lectin EULS3 Is Involved in ABA Signaling in Roots.Frontiers in plant science · 2020Article
- The fungal ribonuclease-like effector protein CSEP0064/BEC1054 represses plant immunity and interferes with degradation of host ribosomal RNA.PLoS pathogens · 2019Article
- Article
- MultiBacMam Bimolecular Fluorescence Complementation (BiFC) tool-kit identifies new small-molecule inhibitors of the CDK5-p25 protein-protein interaction (PPI).Scientific reports · 2018Article
- How to prove the existence of metabolons?Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2018Review
- Chlorophyll Fluorescence Video Imaging: A Versatile Tool for Identifying Factors Related to Photosynthesis.Frontiers in plant science · 2018Review
- Arabidopsis response regulator 22 inhibits cytokinin-regulated gene transcription in vivo.Protoplasma · 2017Article
- Identification and Molecular Characterization of the Chloroplast Targeting Domain ofFrontiers in plant science · 2017Article
- Techniques for the Analysis of Protein-Protein Interactions in Vivo.Plant physiology · 2016Review
- A G-Box-Like Motif Is Necessary for Transcriptional Regulation by Circadian Pseudo-Response Regulators in Arabidopsis.Plant physiology · 2016Article
- Calcium Sensors as Key Hubs in Plant Responses to Biotic and Abiotic Stresses.Frontiers in plant science · 2016Review
- SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants.eLife · 2015Article
- Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic Protein Interaction Studies.Plant physiology · 2015Article
- Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies.Journal of molecular biology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding protein and gene function requires identifying interaction partners using biochemical, molecular or genetic tools. In plants, searching for novel protein-protein interactions is limited to protein purification assays, heterologous in vivo systems such as the yeast-two-hybrid or mutant screens. Ideally one would be able to search for novel protein partners in living plant cells. We demonstrate that it is possible to screen for novel protein-protein interactions from a random library in protoplasted Arabidopsis plant cells and recover some of the interacting partners. Our screen is based on capturing the bi-molecular complementation of mYFP between an YN-bait fusion partner and a completely random prey YC-cDNA library with FACS. The candidate interactions were confirmed using in planta BiFC assays and in planta FRET-FLIM assays. From this work, we show that the well characterized protein Calcium Dependent Protein Kinase 3 (CPK3) interacts with APX3, HMGB5, ORP2A and a ricin B-related lectin domain containing protein At2g39050. This is one of the first randomin planta screens to be successfully employed.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.