ArticleZebrafish2012
Efficient shRNA-mediated inhibition of gene expression in zebrafish.
Article in Zebrafish, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
- A recombinase-activated ribozyme to knock down endogenous gene expression in zebrafish.PLoS genetics · 2025Article
- MicroRNA-9 promotes axon regeneration of mauthner-cell in zebrafish via her6/ calcium activity pathway.Cellular and molecular life sciences : CMLS · 2024Article
- Review: Recent Applications of Gene Editing in Fish Species and Aquatic Medicine.Animals : an open access journal from MDPI · 2023Review
- Plasmid-driven RNA interference in fish cell lines.In vitro cellular & developmental biology. Animal · 2022Article
- A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish.Journal of cell science · 2021Article
- Targeted RNA Knockdown by a Type III CRISPR-Cas Complex in Zebrafish.The CRISPR journal · 2020Article
- Modeling Neuronal Diseases in Zebrafish in the Era of CRISPR.Current neuropharmacology · 2020Review
- NudC regulates photoreceptor disk morphogenesis and rhodopsin localization.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- CaNature communications · 2019Article
- Basal epithelial tissue folding is mediated by differential regulation of microtubules.Development (Cambridge, England) · 2018Article
- A Seed Mismatch Enhances Argonaute2-Catalyzed Cleavage and Partially Rescues Severely Impaired Cleavage Found in Fish.Molecular cell · 2017Article
- Overexpression of microRNA-722 fine-tunes neutrophilic inflammation by inhibitingDisease models & mechanisms · 2017Article
- Zebrafish models in neuropsychopharmacology and CNS drug discovery.British journal of pharmacology · 2017Review
- MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through TargetingFrontiers in molecular neuroscience · 2017Article
- Advancements in zebrafish applications for 21st century toxicology.Pharmacology & therapeutics · 2016Review
- Challenges in understanding psychiatric disorders and developing therapeutics: a role for zebrafish.Disease models & mechanisms · 2015Review
- Effective heritable gene knockdown in zebrafish using synthetic microRNAs.Nature communications · 2015Article
- Review
- Nucleotide bound to rab11a controls localization in rod cells but not interaction with rhodopsin.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Modelling inborn errors of metabolism in zebrafish.Journal of inherited metabolic disease · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Despite the broad repertoire of loss of function (LOF) tools available for use in the zebrafish, there remains a need for a simple and rapid method that can inhibit expression of genes at later stages. RNAi would fulfill that role, and a previous report (Dong et al. 2009) provided encouraging data. The goal of this study was to further address the ability of expressed shRNAs to inhibit gene expression. This included quantifying RNA knockdown, testing specificity of shRNA effects, and determining whether tissue-specific LOF could be achieved. Using an F0 transgenic approach, this report demonstrates that for two genes, wnt5b and zDisc1, each with described mutant and morphant phenotypes, shRNAs efficiently decrease endogenous RNA levels. Phenotypes elicited by shRNA resemble those of mutants and morphants, and are reversed by expression of cognate RNA, further demonstrating specificity. Tissue-specific expression of zDisc1 shRNAs in F0 transgenics demonstrates that conditional LOF can be readily obtained. These results suggest that shRNA expression presents a viable approach for rapid inhibition of zebrafish gene expression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.