ArticleMolecular & cellular proteomics : MCP2012
System response of metabolic networks in Chlamydomonas reinhardtii to total available ammonium.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 101 citations in OpenAlex.
- Integrative metabolomics reveals unique metabolic traits in Guillain-Barré Syndrome and its variants.Scientific reports · 2019Trial
- Metabolic rewiring and biomass redistribution enable optimized mixotrophic growth in Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- From Feasting to Fasting: The Arginine Pathway as a Metabolic Switch in Nitrogen-DeprivedCells · 2023Review
- Development of a stable semi-continuous lipid production system of an oleaginous Chlamydomonas sp. mutant using multi-omics profiling.Biotechnology for biofuels and bioproducts · 2022Article
- Photosynthesis and pigment production: elucidation of the interactive effects of nutrients and light on Chlamydomonas reinhardtii.Bioprocess and biosystems engineering · 2022Article
- Metabolomics of Chlorophylls and Carotenoids: Analytical Methods and Metabolome-Based Studies.Antioxidants (Basel, Switzerland) · 2021Review
- Algal glycobiotechnology: omics approaches for strain improvement.Microbial cell factories · 2021Review
- Large fluxes of fatty acids from membranes to triacylglycerol and back during N-deprivation and recovery in Chlamydomonas.Plant physiology · 2021Article
- Plant Single-Cell Metabolomics-Challenges and Perspectives.International journal of molecular sciences · 2020Review
- Surviving Starvation: Proteomic and Lipidomic Profiling of Nutrient Deprivation in the Smallest Known Free-Living Eukaryote.Metabolites · 2020Article
- Targeted Metabolomic and Biochemical Changes During Nitrogen Stress Mediated Lipid Accumulation inFrontiers in bioengineering and biotechnology · 2020Article
- Remodeling of Chlamydomonas Metabolism Using Synthetic Inducers Results in Lipid Storage during Growth.Plant physiology · 2019Article
- FermentedNutrients · 2019Article
- Increased urea availability promotes adjustments in C/N metabolism and lipid content without impacting growth in Chlamydomonas reinhardtii.Metabolomics : Official journal of the Metabolomic Society · 2019Article
- Article
- Functional photosystem I maintains proper energy balance during nitrogen depletion inBiotechnology for biofuels · 2017Article
- MicroRNAs modulate adaption to multiple abiotic stresses in Chlamydomonas reinhardtii.Scientific reports · 2016Article
- Nutrient removal by Chlorella vulgaris F1068 under cetyltrimethyl ammonium bromide induced hormesis.Environmental science and pollution research international · 2016Article
- Proteome response ofAlgal research · 2016Article
- Metabolomics by Gas Chromatography-Mass Spectrometry: Combined Targeted and Untargeted Profiling.Current protocols in molecular biology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drastic alterations in macronutrients are known to cause large changes in biochemistry and gene expression in the photosynthetic alga Chlamydomonas reinhardtii. However, metabolomic and proteomic responses to subtle reductions in macronutrients have not yet been studied. When ammonium levels were reduced by 25-100% compared with control cultures, ammonium uptake and growth rates were not affected at 25% or 50% nitrogen-reduction for 28 h. However, primary metabolism and enzyme expression showed remarkable changes at acute conditions (4 h and 10 h after ammonium reduction) compared with chronic conditions (18 h and 28 h time points). Responses of 145 identified metabolites were quantified using gas chromatography-time of flight mass spectrometry; 495 proteins (including 187 enzymes) were monitored using liquid chromatography-ion trap mass spectrometry with label-free spectral counting. Stress response and carbon assimilation processes (Calvin cycle, acetate uptake and chlorophyll biosynthesis) were altered first, in addition to increase in enzyme contents for lipid biosynthesis and accumulation of short chain free fatty acids. Nitrogen/carbon balance metabolism was found changed only under chronic conditions, for example in the citric acid cycle and amino acid metabolism. Metabolism in Chlamydomonas readily responds to total available media nitrogen with temporal increases in short-chain free fatty acids and turnover of internal proteins, long before nitrogen resources are depleted.
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Registered trials
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