ArticleJournal of virology2012
Identification of human astrovirus genome-linked protein (VPg) essential for virus infectivity.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 67 citations in OpenAlex.
- Viral protease-mediated polyprotein processing in human astroviruses.Npj viruses · 2026Article
- Three-dimensional peptide modeling and subcellular localization of the VPg protein of human astrovirus 8 in infected Caco-2 cells.Molecular biology reports · 2026Article
- Complex Recombination Landscape and Lineage Turnover in Classical Human Astroviruses.Microorganisms · 2026Article
- Review
- The dynamics and strategy of RNA replication in astroviruses.NAR molecular medicine · 2026Article
- Identification of viral protease-dependent cleavage sites within the human astrovirus polyprotein.Journal of virology · 2025Article
- Rapid Rescue of Goose Astrovirus Genome via Red/ET Assembly.Food and environmental virology · 2024Article
- The astrovirus N-terminal nonstructural protein anchors replication complexes to the perinuclear ER membranes.PLoS pathogens · 2024Article
- Article
- Diversity of classic and novel human astrovirus in outpatient children with acute gastroenteritis in Shanghai, China.Frontiers in microbiology · 2023Article
- Diversity of human astroviruses in Germany 2018 and 2019.Virology journal · 2022Article
- Maize Lethal Necrosis disease: review of molecular and genetic resistance mechanisms, socio-economic impacts, and mitigation strategies in sub-Saharan Africa.BMC plant biology · 2022Review
- Multiple Viral Protein Genome-Linked Proteins Compensate for Viral Translation in a Positive-Sense Single-Stranded RNA Virus Infection.Journal of virology · 2022Article
- Article
- Protein Nucleotidylylation in +ssRNA Viruses.Viruses · 2021Review
- Intra- and Cross-Species Transmission of Astroviruses.Viruses · 2021Review
- A hidden gene in astroviruses encodes a viroporin.Nature communications · 2020Article
- Fecal Components Modulate Human Astrovirus Infectivity in Cells and Reconstituted Intestinal Tissues.mSphere · 2019Article
- A novel group of avian astroviruses from Neotropical passerine birds broaden the diversity and host range of Astroviridae.Scientific reports · 2019Article
- Human astroviruses: in silico analysis of the untranslated region and putative binding sites of cellular proteins.Molecular biology reports · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viral genome-linked proteins (VPgs) have been identified in several single-stranded positive-sense RNA virus families. The presence of such protein in the family Astroviridae has not been fully elucidated, although a putative VPg coding region in open reading frame 1a (ORF1a) of astrovirus with high amino acid sequence similarity to the VPg coding region of Caliciviridae has been previously identified. In this work we present several experimental findings that show that human astrovirus (HAstV) RNA encodes a VPg essential for viral infectivity: (i) RNase treatment of RNA purified from astrovirus-infected cells results in a single protein of 13 to 15 kDa, compatible with the predicted astrovirus VPg size; (ii) the antibody used to detect this 13- to 15-kDa protein is specifically directed against a region that includes the putative VPg coding region; (iii) the 13- to 15-kDa protein detected has been partially sequenced and the sequence obtained is contained in the computationally predicted VPg; (iv) the protein resulting from this putative VPg coding region is a highly disordered protein, resembling the VPg of sobemo-, calici- and potyviruses; (v) proteolytic treatment of the genomic RNA leads to loss of infectivity; and (vi) mutagenesis of Tyr-693 included in the putative VPg protein is lethal for HAstV replication, which strongly supports its functional role in the covalent link with the viral RNA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.