ArticlePlant physiology2012
Rice mitogen-activated protein kinase interactome analysis using the yeast two-hybrid system.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Genome-wide identification of the MAPK gene family in peanut (Arachis hypogaea L.) and functional characterization of AhMPK3 and AhMPK18 in plant innate immunity.BMC plant biology · 2026Article
- Rice blast pathogen effector AvrPib compromises disease resistance by targeting Raf-like protein kinase OsMAPKKK72 to inhibit MAPK signaling.Journal of integrative plant biology · 2026Article
- Ferroptosis in plant immunity.Plant communications · 2025Review
- Global Protein Interactome Mapping in Rice Using Barcode-Indexed PCR Coupled with HiFi Long-Read Sequencing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Genome-Wide Identification of MKK Gene Family and Response to Hormone and Abiotic Stress in Rice.Plants (Basel, Switzerland) · 2024Article
- Mitogen-Activated Protein Kinase Kinase OsMEK2 Positively Regulates CaAntioxidants (Basel, Switzerland) · 2024Article
- OsMKK1 is a novel element that positively regulates the Xa21-mediated resistance response to Xanthomonas oryzae pv. oryzae in rice.Plant cell reports · 2024Article
- OsMAPK6 positively regulates rice cold tolerance at seedling stage via phosphorylating and stabilizing OsICE1 and OsIPA1.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Identification and Expression Analysis of MPK and MKK Gene Families in Pecan (International journal of molecular sciences · 2022Article
- The NIN-Like Protein OsNLP2 Negatively Regulates Ferroptotic Cell Death and Immune Responses toAntioxidants (Basel, Switzerland) · 2022Article
- Rice calcium/calmodulin-dependent protein kinase directly phosphorylates a mitogen-activated protein kinase kinase to regulate abscisic acid responses.The Plant cell · 2021Article
- Update on the Roles of Rice MAPK Cascades.International journal of molecular sciences · 2021Review
- Comparative analysis ofPeerJ · 2021Article
- Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice-Frontiers in plant science · 2021Article
- Differential expression of MEKK subfamily genes inPlant signaling & behavior · 2020Article
- The MAPK substrate MASS proteins regulate stomatal development in Arabidopsis.PLoS genetics · 2020Article
- Proteomic characterization of MPK4 signaling network and putative substrates.Plant molecular biology · 2019Article
- Phosphorylation regulates the activity of INDETERMINATE-DOMAIN (IDD/BIRD) proteins in response to diverse environmental conditions.Plant signaling & behavior · 2019Review
- Identification on mitogen-activated protein kinase signaling cascades by integrating protein interaction with transcriptional profiling analysis in cotton.Scientific reports · 2018Article
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13 authors.
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Abstract
Mitogen-activated protein kinase (MAPK) cascades support the flow of extracellular signals to intracellular target molecules and ultimately drive a diverse array of physiological functions in cells, tissues, and organisms by interacting with other proteins. Yet, our knowledge of the global physical MAPK interactome in plants remains largely fragmented. Here, we utilized the yeast two-hybrid system and coimmunoprecipitation, pull-down, bimolecular fluorescence complementation, subcellular localization, and kinase assay experiments in the model crop rice (Oryza sativa) to systematically map what is to our knowledge the first plant MAPK-interacting proteins. We identified 80 nonredundant interacting protein pairs (74 nonredundant interactors) for rice MAPKs and elucidated the novel proteome-wide network of MAPK interactors. The established interactome contains four membrane-associated proteins, seven MAP2Ks (for MAPK kinase), four MAPKs, and 59 putative substrates, including 18 transcription factors. Several interactors were also validated by experimental approaches (in vivo and in vitro) and literature survey. Our results highlight the importance of OsMPK1, an ortholog of tobacco (Nicotiana benthamiana) salicyclic acid-induced protein kinase and Arabidopsis (Arabidopsis thaliana) AtMPK6, among the rice MAPKs, as it alone interacts with 41 unique proteins (51.2% of the mapped MAPK interaction network). Additionally, Gene Ontology classification of interacting proteins into 34 functional categories suggested MAPK participation in diverse physiological functions. Together, the results obtained essentially enhance our knowledge of the MAPK-interacting protein network and provide a valuable research resource for developing a nearly complete map of the rice MAPK interactome.
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