ArticleThe Journal of surgical research2012
Rotavirus infection of human cholangiocytes parallels the murine model of biliary atresia.
Article in The Journal of surgical research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Correlation analyses of ultrasonographic and histopathological characteristics of porta hepatis lymph nodes in biliary atresia.BMC gastroenterology · 2025Article
- Multiple susceptibility enhancer variants increasingFrontiers in genetics · 2025Article
- Specific binding sites on Rhesus rotavirus capsid protein dictate the method of endocytosis inducing the murine model of biliary atresia.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Long non-coding RNA growth arrest-specific 5 inhibits liver fibrogenesis in biliary atresia by interacting with microRNA-222 and repressing IGF1/AKT signaling.Translational pediatrics · 2023Article
- Biliary Atresia Animal Models: Is the Needle in a Haystack?International journal of molecular sciences · 2022Review
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- Innate Immunity and Pathogenesis of Biliary Atresia.Frontiers in immunology · 2020Review
- Prominin-1 Promotes Biliary Fibrosis Associated With Biliary Atresia.Hepatology (Baltimore, Md.) · 2019Article
- A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice.Journal of visualized experiments : JoVE · 2018Article
- Non-Human Primate Models of Enteric Viral Infections.Viruses · 2018Review
- Vesicle-Cloaked Virus Clusters Are Optimal Units for Inter-organismal Viral Transmission.Cell host & microbe · 2018Article
- The SRL peptide of rhesus rotavirus VP4 protein governs cholangiocyte infection and the murine model of biliary atresia.Hepatology (Baltimore, Md.) · 2017Article
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- Rhesus rotavirus VP4 sequence-specific activation of mononuclear cells is associated with cholangiopathy in murine biliary atresia.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Functional and structural features of cholangiocytes in health and disease.Cellular and molecular gastroenterology and hepatology · 2015Article
- Rotavirus Infects Human Biliary Epithelial Cells and Stimulates Secretion of Cytokines IL-6 and IL-8 via MAPK Pathway.BioMed research international · 2015Article
- Expansion of prominin-1-expressing cells in association with fibrosis of biliary atresia.Hepatology (Baltimore, Md.) · 2014Article
- Role of myeloid differentiation factor 88 in Rhesus rotavirus-induced biliary atresia.The Journal of surgical research · 2013Article
- Rotavirus replication in the cholangiocyte mediates the temporal dependence of murine biliary atresia.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
introductionBiliary atresia (BA) is the leading indication for liver transplantation in the pediatric population. The murine model of BA supports a viral etiology, because infection of neonatal mice with rhesus rotavirus (RRV) results in biliary obstruction. Viral infection targets the biliary epithelium and development of the model is viral strain dependent. No study has yet determined whether human cholangiocytes are also susceptible to rotaviral infection. We established an in vitro human model using an immortalized human cholangiocyte cell line and primary human cholangiocytes obtained from explanted livers to determine human cholangiocyte susceptibility to rotavirus infection.
methodsReplication and binding assays were performed on immortalized mouse (mCL) and human (H69) cells using six different strains of rotavirus. Primary human cholangiocytes were isolated from cadaveric livers, characterized in culture, and infected with RRV, which causes BA in mice, and another simian strain, TUCH, which does not cause BA in mice.
resultsImmortalized mouse and human cholangiocytes demonstrated similar patterns of infectivity and binding with different strains of rotavirus. Both cell lines produced a significantly higher viral yield with RRV infection than with the other strains tested. In primary human cholangiocytes, which maintained their epithelial characteristics, as demonstrated by cytokeratin staining, RRV replicated to a yield 1000-fold higher than TUCH.
conclusionsBoth immortalized and primary human cholangiocytes are susceptible to RRV infection in a fashion similar to murine cholangiocytes. These novel findings suggest rotavirus infection could have a potential role in the pathogenesis of human BA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.