ArticleJournal of clinical microbiology2012
Barcode identifiers as a practical tool for reliable species assignment of medically important black yeast species.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- SystemicFrontiers in veterinary science · 2023Article
- Phaeohyphomycosis due toPathogens (Basel, Switzerland) · 2022Article
- Environmental prospecting of black yeast-like agents of human disease using culture-independent methodology.Scientific reports · 2020Article
- Comparison of the performance of ITS1 and ITS2 as barcodes in amplicon-based sequencing of bioaerosols.PeerJ · 2020Article
- A NovelFrontiers in veterinary science · 2020Article
- Review
- New Molecular Markers Distinguishing Fonsecaea Agents of Chromoblastomycosis.Mycopathologia · 2019Article
- Biodiversity and human-pathogenicity ofPersoonia · 2017Article
- Cladophialophora bantiana osteomyelitis in a renal transplant patient.Medical mycology case reports · 2016Article
- ATR-FTIR Spectroscopy Highlights the Problem of Distinguishing Between Exophiala dermatitidis and E. phaeomuriformis Using MALDI-TOF MS.Microbial ecology · 2016Article
- One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes.Persoonia · 2015Article
- Morphological and Molecular Characterization of Exophiala polymorpha sp. nov. Isolated from Sporotrichoid Lymphocutaneous Lesions in a Patient with Myasthenia Gravis.Journal of clinical microbiology · 2015Article
- In vitro activities of eight antifungal drugs against 106 waterborne and cutaneous exophiala species.Antimicrobial agents and chemotherapy · 2013Article
- A case of Exophiala oligosperma successfully treated with voriconazole.Medical mycology case reports · 2013Article
- Analysis of black fungal biofilms occurring at domestic water taps. II: potential routes of entry.Mycopathologia · 2013Article
- Analysis of black fungal biofilms occurring at domestic water taps. I: compositional analysis using Tag-Encoded FLX Amplicon Pyrosequencing.Mycopathologia · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Herpotrichiellaceous black yeasts and relatives comprise severe pathogens flanked by nonpathogenic environmental siblings. Reliable identification by conventional methods is notoriously difficult. Molecular identification is hampered by the sequence variability in the internal transcribed spacer (ITS) domain caused by difficult-to-sequence homopolymeric regions and by poor taxonomic attribution of sequences deposited in GenBank. Here, we present a potential solution using short barcode identifiers (27 to 50 bp) based on ITS2 ribosomal DNA (rDNA), which allows unambiguous definition of species-specific fragments. Starting from proven sequences of ex-type and authentic strains, we were able to describe 103 identifiers. Multiple BLAST searches of these proposed barcode identifiers in GenBank revealed uniqueness for 100 taxonomic entities, whereas the three remaining identifiers each matched with two entities, but the species of these identifiers could easily be discriminated by differences in the remaining ITS regions. Using the proposed barcode identifiers, a 4.1-fold increase of 100% matches in GenBank was achieved in comparison to the classical approach using the complete ITS sequences. The proposed barcode identifiers will be made accessible for the diagnostic laboratory in a permanently updated online database, thereby providing a highly practical, reliable, and cost-effective tool for identification of clinically important black yeasts and relatives.
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