ArticleDNA repair2012
Escherichia coli UmuC active site mutants: effects on translesion DNA synthesis, mutagenesis and cell survival.
Article in DNA repair, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Acinetobacter baumannii encodes multiple mutagenically and biochemically active DNA polymerase V variants.Nucleic acids research · 2026Article
- Persistent ciprofloxacin exposure induced the transformation of Klebsiella pneumoniae small colony variant into mucous phenotype.Frontiers in cellular and infection microbiology · 2023Article
- Development of Resistance inAntibiotics (Basel, Switzerland) · 2020Article
- Role of RNase H enzymes in maintaining genome stability in Escherichia coli expressing a steric-gate mutant of pol VDNA repair · 2019Article
- High genomic variability in the plant pathogenic bacterium Pectobacterium parmentieri deciphered from de novo assembled complete genomes.BMC genomics · 2018Article
- Ribonucleotide discrimination by translesion synthesis DNA polymerases.Critical reviews in biochemistry and molecular biology · 2018Review
- Altering the N-terminal arms of the polymerase manager protein UmuD modulates protein interactions.PloS one · 2017Article
- Unlocking the steric gate of DNA polymerase η leads to increased genomic instability in Saccharomyces cerevisiae.DNA repair · 2015Article
- Redundancy in ribonucleotide excision repair: Competition, compensation, and cooperation.DNA repair · 2015Review
- Ribonucleotides in bacterial DNA.Critical reviews in biochemistry and molecular biology · 2015Review
- Investigating the mechanisms of ribonucleotide excision repair in Escherichia coli.Mutation research · 2014Article
- Variants of mouse DNA polymerase κ reveal a mechanism of efficient and accurate translesion synthesis past a benzo[a]pyrene dG adduct.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Polynucleobacter necessarius, a model for genome reduction in both free-living and symbiotic bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Removal of misincorporated ribonucleotides from prokaryotic genomes: an unexpected role for nucleotide excision repair.PLoS genetics · 2013Article
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- Mechanisms employed by Escherichia coli to prevent ribonucleotide incorporation into genomic DNA by Pol V.PLoS genetics · 2012Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Escherichia coli polymerase V (pol V/UmuD(2)'C) is a low-fidelity DNA polymerase that has recently been shown to avidly incorporate ribonucleotides (rNTPs) into undamaged DNA. The fidelity and sugar selectivity of pol V can be modified by missense mutations around the "steric gate" of UmuC. Here, we analyze the ability of three steric gate mutants of UmuC to facilitate translesion DNA synthesis (TLS) of a cyclobutane pyrimidine dimer (CPD) in vitro, and to promote UV-induced mutagenesis and cell survival in vivo. The pol V (UmuC_F10L) mutant discriminates against rNTP and incorrect dNTP incorporation much better than wild-type pol V and although exhibiting a reduced ability to bypass a CPD in vitro, does so with high-fidelity and consequently produces minimal UV-induced mutagenesis in vivo. In contrast, pol V (UmuC_Y11A) readily misincorporates both rNTPs and dNTPs during efficient TLS of the CPD in vitro. However, cells expressing umuD'C(Y11A) were considerably more UV-sensitive and exhibited lower levels of UV-induced mutagenesis than cells expressing wild-type umuD'C or umuD'C(Y11F). We propose that the increased UV-sensitivity and reduced UV-mutability of umuD'C(Y11A) is due to excessive incorporation of rNTPs during TLS that are subsequently targeted for repair, rather than an inability to traverse UV-induced lesions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.