ArticlePlant methods2012
Robust transformation procedure for the production of transgenic farmer-preferred cassava landraces.
Article in Plant methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Optimizing somatic embryogenesis in Indonesian cassava genotypes: Effects of 2,4-D or Picloram supplementation and explant type.Plant biotechnology (Tokyo, Japan) · 2026Article
- Improving gene editing of CRISPR/Cas9 using the callus-specific promoterFrontiers in plant science · 2025Article
- CRISPR/Cas9: an advanced platform for root and tuber crops improvement.Frontiers in genome editing · 2023Review
- CC-type glutaredoxin, MeGRXC3, associates with catalases and negatively regulates drought tolerance in cassava (Manihot esculenta Crantz).Plant biotechnology journal · 2022Article
- Article
- Cassava mosaic disease and its management in Southeast Asia.Plant molecular biology · 2022Review
- CRISPR-Cas9-mediated knockout ofFrontiers in plant science · 2022Article
- Studies on Pure MlbFrontiers in plant science · 2022Article
- Efficient Genetic Transformation and Regeneration of a Farmer-Preferred Cassava Cultivar From Ghana.Frontiers in plant science · 2021Article
- A cassava protoplast system for screening genes associated with the response to South African cassava mosaic virus.Virology journal · 2020Article
- RNA silencing ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Genome Editing in Agriculture: Technical and Practical Considerations.International journal of molecular sciences · 2019Review
- ArabidopsisFrontiers in plant science · 2019Article
- Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels.Methods and protocols · 2018Article
- Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots.Plant methods · 2018Article
- Novel Bioengineered Cassava Expressing an Archaeal Starch Degradation System and a Bacterial ADP-Glucose Pyrophosphorylase for Starch Self-Digestibility and Yield Increase.Frontiers in plant science · 2018Article
- RNAi inhibition of feruloyl CoA 6'-hydroxylase reduces scopoletin biosynthesis and post-harvest physiological deterioration in cassava (Manihot esculenta Crantz) storage roots.Plant molecular biology · 2017Article
- Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz).BMC biotechnology · 2017Article
- Gene expression atlas for the food security crop cassava.The New phytologist · 2017Article
- Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate.PloS one · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent progress in cassava transformation has allowed the robust production of transgenic cassava even under suboptimal plant tissue culture conditions. The transformation protocol has so far been used mostly for the cassava model cultivar 60444 because of its good regeneration capacity of embryogenic tissues. However, for deployment and adoption of transgenic cassava in the field it is important to develop robust transformation methods for farmer- and industry-preferred landraces and cultivars. Because dynamics of multiplication and regeneration of embryogenic tissues differ between cassava genotypes, it was necessary to adapt the efficient cv. 60444 transformation protocol to genotypes that are more recalcitrant to transformation. Here we demonstrate that an improved cassava transformation protocol for cv. 60444 could be successfully modified for production of transgenic farmer-preferred cassava landraces. The modified transformation method reports on procedures for optimization and is likely transferable to other cassava genotypes reportedly recalcitrant to transformation provided production of high quality FEC. Because the three farmer-preferred cassava landraces selected in this study have been identified as resistant or tolerant to cassava mosaic disease (CMD), the adapted protocol will be essential to mobilize improved traits into cassava genotypes suitable for regions where CMD limits production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.