ArticleProceedings of the National Academy of Sciences of the United States of America2012
Effects of a single nucleotide polymorphism in the chicken NK-lysin gene on antimicrobial activity and cytotoxicity of cancer cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 48 citations in OpenAlex.
- Effect of Nk-lysin peptides on bacterial growth, MIC, antimicrobial resistance, and viral activities.Animal biotechnology · 2024Article
- From oncolytic peptides to oncolytic polymers: A new paradigm for oncotherapy.Bioactive materials · 2024Review
- Genomic Variant in NK-Lysin Gene Is Associated with T Lymphocyte Subpopulations in Pigs.Genes · 2022Article
- Response of immunoglobulin M in gut mucosal immunity of common carp (Frontiers in immunology · 2022Article
- Functional Characterization of Porcine NK-Lysin: A Novel Immunomodulator That Regulates Intestinal Inflammatory Response.Molecules (Basel, Switzerland) · 2021Article
- Induction of Chicken Host Defense Peptides within Disease-Resistant and -Susceptible Lines.Genes · 2020Article
- Impact of energy restriction during late gestation on the muscle and blood transcriptome of beef calves after preconditioning.BMC genomics · 2018Article
- AvBD1 nucleotide polymorphisms, peptide antimicrobial activities and microbial colonisation of the broiler chicken gut.BMC genomics · 2017Article
- Tissue expression and antibacterial activity of host defense peptides in chicken.BMC veterinary research · 2016Article
- Composite Membranes of Recombinant Silkworm Antimicrobial Peptide and Poly (L-lactic Acid) (PLLA) for biomedical application.Scientific reports · 2016Article
- Expression of the Bovine NK-Lysin Gene Family and Activity against Respiratory Pathogens.PloS one · 2016Article
- Mapping and genotypic analysis of the NK-lysin gene in chicken.Genetics, selection, evolution : GSE · 2014Article
- Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NK-lysin is an effector protein of the innate immune system and an important component of host protection. We isolated a SNP in the NK-lysin coding sequence among different chicken breeds. This A to G substitution at the position 271 nucleotide in the ORF results in an Asn (N) to Asp (D) amino acid alteration. We synthesized two 30-aa peptides (N29N and N29D) to compare the biological activity of the helix 2-loop-helix 3 region of NK-lysin resulting from the polymorphic gene. Both peptides were found to be cytotoxic in bacteria and tumor cell cultures at micromolar concentrations. The N29N peptide, however, exhibited greater antibacterial and anticancer activity than the N29D peptide. Circular dichroism spectroscopy of the two peptides in negatively charged single unilamellar vesicles showed spectra typical of α-helical peptides. The helical profile of N29D was reduced substantially compared with that of N29N. However, no structural change was observed in neutral vesicles. ζ-Potential measurements of liposomes incubated with increasing peptide concentrations allowed surface charge neutralization with a negatively charged lipid, but not with a zwitterionic lipid. This result suggests that a difference in electrostatic interaction between lipid membranes and the helical peptides results from the polymorphic gene and is subsequently an important factor in cell lytic activity of variant NK-lysin peptides.
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