ArticleParasitology research2012
In vitro human cell-free expression system for synthesis of malaria proteins.
Article in Parasitology research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 4 citations in OpenAlex.
- Cell-Free Systems and Their Importance in the Study of Membrane Proteins.The Journal of membrane biology · 2025Review
- HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins.Journal of visualized experiments : JoVE · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we performed cell-free expression of Plasmodium proteins using the in vitro human cell-free protein expression systems for DNA and mRNA. Malaria rhoptry genes (PFc14_0344, PFc0120w, PY01759, PY00763, PY07482, and PY04666) and a Maurer's cleft gene (PfA0680c) identified from proteome analysis studies were cloned into the pT7CFE1-CHis expression vector. Following a coupled transcription-translation procedure, expressed proteins were analyzed by His-tag staining and by western blotting using protein specific antibodies and nickel-horseradish peroxidase (HRP) for histidine detection. Antibodies against whole rhoptries of Plasmodium falciparum and Plasmodium yoelii merozoites and antibodies specific for the PfMC-2TM protein identified translated proteins. The rhoptry specific antibodies exhibited cross reactivity among the expressed proteins of P. falciparum and P. yoelii. The results demonstrate that the in vitro human cell-free protein expression system is suitable for rapid expression and screening of malaria vaccine candidates and diagnostic biomarkers.
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