ArticleCytotechnology2013
PEI/DNA formation affects transient gene expression in suspension Chinese hamster ovary cells via a one-step transfection process.
Article in Cytotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- AAV vector production in suspension cells using PEI transfection and sodium butyrate with orthogonal assessment of function and quality.Molecular therapy. Advances · 2026Article
- Surface-Capped Protein Nanoparticles for Nonviral Gene Delivery.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- An effective method to enhance transient gene expression by transfecting high-density CHO cells.AMB Express · 2025Article
- Revealing two distinct molecular binding modes in polyethyleneimine-DNA polyplexes using infrared spectroscopy.Soft matter · 2025Article
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- A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M.Fish and shellfish immunology reports · 2023Article
- A feasibility study of different commercially available serum-free mediums to enhance lentivirus and adeno-associated virus production in HEK 293 suspension cells.Cytotechnology · 2022Article
- Fluorescent labeling of plasmid DNA for gene delivery: Implications of dye hydrophobicity on labeling efficiencies and nanoparticle size.Analytical biochemistry · 2022Article
- Augmenting recombinant antibody production in HEK293E cells: optimizing transfection and culture parameters.Antibody therapeutics · 2022Article
- Poly(Beta-Amino Ester)s as High-Yield Transfection Reagents for Recombinant Protein Production.International journal of nanomedicine · 2022Article
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- Oral Delivery of Nucleic Acids with Passive and Active Targeting to the Intestinal Tissue Using Polymer-Based Nanocarriers.Pharmaceutics · 2021Article
- Mechanistic model for production of recombinant adeno-associated virus via triple transfection of HEK293 cells.Molecular therapy. Methods & clinical development · 2021Article
- Article
- Article
- Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A VHH-Based Anti-MUC1 Chimeric Antigen Receptor for Specific Retargeting of Human Primary T Cells to MUC1-Positive Cancer Cells.Cell journal · 2021Article
- Tuning the Surface Charge of Self-Assembled Polydiacetylene Vesicles to Control Aggregation and Cell Binding.Biosensors · 2020Article
- High cell density transient transfection of CHO cells for TGF-β1 expression.Engineering in life sciences · 2019Article
- Functional characterization of a SNP (F51S) found in human alpha 1-antitrypsin.Molecular genetics & genomic medicine · 2019Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyethylenimine (PEI) has been used widely in transient gene expression studies of mammalian cells. We performed transient gene expression in suspension Chinese hamster ovary cells using a one-step transfection procedure in which DNA and PEI were simultaneously added to a cell culture in suspension without prior PEI/DNA complex incubation. To further understand the effect of PEI/DNA formation on the transfection and expression of exogenous gene in shaking state, we investigated the diameter and overcharge of the PEI/DNA complex. The results showed that the diameter of the complex was smaller with more positive charge when the PEI/DNA ratio was higher. Moreover, DNA more easily penetrated cells and nuclei at higher PEI concentrations. The highest transcription level, transfection efficiency, and GFP expression were obtained when the PEI/DNA ratio was 5:1.
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