ArticleJournal of visualized experiments : JoVE2012
Hybridization in situ of salivary glands, ovaries, and embryos of vector mosquitoes.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Thermal sensitivity in a symbiont: effect of elevated cyclic temperature exposure on Wolbachia density in Culex quinquefasciatus (Diptera: Culicidae) embryos.Journal of insect science (Online) · 2026Article
- Unveiling the developmental dynamics and functional role of Odorant Receptor Co-receptor (Orco) in Aedes albopictus: A novel mechanism for regulating odorant receptor expression.PLoS neglected tropical diseases · 2025Article
- Single-cell transcriptional landscapes of Aedes aegypti midgut and fat body after a bloodmeal.Cell genomics · 2025Article
- Maternal lipid mobilization is essential for embryonic development in the malaria vector Anopheles gambiae.PLoS biology · 2024Article
- The mechanism of cytoplasmic incompatibility is conserved in Wolbachia-infected Aedes aegypti mosquitoes deployed for arbovirus control.PLoS biology · 2024Article
- Article
- Precise coordination between nutrient transporters ensures fertility in the malaria mosquito Anopheles gambiae.PLoS genetics · 2024Article
- Fertility decline in female mosquitoes is regulated by theiScience · 2023Article
- A DBHS family member regulates male determination in the filariasis vector Armigeres subalbatus.Nature communications · 2023Article
- Anterior-posterior patterning of segments in Anopheles stephensi offers insights into the transition from sequential to simultaneous segmentation in holometabolous insects.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2023Article
- CRISPR mediated transactivation in the human disease vector Aedes aegypti.PLoS pathogens · 2023Article
- Vitellogenin genes are transcribed in Culex quinquefasciatus ovary.Memorias do Instituto Oswaldo Cruz · 2023Article
- Article
- piRNA pathway gene expression in the malaria vector mosquito Anopheles stephensi.Insect molecular biology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mosquitoes are vectors for a diverse set of pathogens including arboviruses, protozoan parasites and nematodes. Investigation of transcripts and gene regulators that are expressed in tissues in which the mosquito host and pathogen interact, and in organs involved in reproduction are of great interest for strategies to reduce mosquito-borne disease transmission and disrupt egg development. A number of tools have been employed to study and validate the temporal and tissue-specific regulation of gene expression. Here, we describe protocols that have been developed to obtain spatial information, which enhances our understanding of where specific genes are expressed and their products accumulate. The protocol described has been used to validate expression and determine accumulation patterns of transcripts in tissues related to mosquito-borne pathogen transmission, such as female salivary glands, as well as subcellular compartments of ovaries and embryos, which relate to mosquito reproduction and development. The following procedures represent an optimized methodology that improves the efficiency of various steps in the protocol without loss of target-specific hybridization signals. Guidelines for RNA probe preparation, dissection of soft tissues and the general procedure for fixation and hybridization are described in Part A, while steps specific for the collection, fixation, pre-hybridization and hybridization of mosquito embryos are detailed in Part B.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.