ArticleJournal of nucleic acids2012
Small RNA expression profiling by high-throughput sequencing: implications of enzymatic manipulation.
Article in Journal of nucleic acids, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
- A method of comprehensive sequencing analysis of the small RNA fragmentome (RiboMarker).RNA (New York, N.Y.) · 2026Article
- MicroRNAs in Plant Genetic Regulation of Drought Tolerance and Their Function in Enhancing Stress Adaptation.Plants (Basel, Switzerland) · 2025Review
- PIPETS: a statistically informed, gene-annotation agnostic analysis method to study bacterial termination using 3'-end sequencing.BMC bioinformatics · 2024Article
- PIPETS: A statistically informed, gene-annotation agnostic analysis method to study bacterial termination using 3'-end sequencing.bioRxiv : the preprint server for biology · 2024Article
- Isolation and Library Preparation of Planarian piRNAs.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The role of noncoding RNAs in Parkinson's disease: biomarkers and associations with pathogenic pathways.Journal of biomedical science · 2021Review
- cyPhyRNA-seq: a genome-scale RNA-seq method to detect active self-cleaving ribozymes by capturing RNAs with 2',3'RNA biology · 2021Article
- Small RNA-Sequencing: Approaches and Considerations for miRNA Analysis.Diagnostics (Basel, Switzerland) · 2021Review
- Small RNAs Are Implicated in Regulation of Gene and Transposable Element Expression in the Protist Trichomonas vaginalis.mSphere · 2021Article
- A Small RNA-Seq Protocol with Less Bias and Improved Capture of 2'-O-Methyl RNAs.Methods in molecular biology (Clifton, N.J.) · 2021Article
- DTA-SiST: de novo transcriptome assembly by using simplified suffix trees.BMC bioinformatics · 2019Article
- Extracellular miRNAs: From Biomarkers to Mediators of Physiology and Disease.Cell metabolism · 2019Review
- The Promise of next generation sequencing micro RNA for the discovery of new targets in contrast induced acute kidney injury.Annals of translational medicine · 2019Article
- Decreasing miRNA sequencing bias using a single adapter and circularization approach.Genome biology · 2018Article
- Stage-dependent piRNAs in chicken implicated roles in modulating male germ cell development.BMC genomics · 2018Article
- Capturing the 'ome': the expanding molecular toolbox for RNA and DNA library construction.Nucleic acids research · 2018Review
- Systematic comparison of small RNA library preparation protocols for next-generation sequencing.BMC genomics · 2018Article
- Coding and noncoding landscape of extracellular RNA released by human glioma stem cells.Nature communications · 2017Article
- Toward reliable biomarker signatures in the age of liquid biopsies - how to standardize the small RNA-Seq workflow.Nucleic acids research · 2016Article
- Sources and Functions of Extracellular Small RNAs in Human Circulation.Annual review of nutrition · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic regulatory small RNAs (sRNAs) play significant roles in many fundamental cellular processes. As such, they have emerged as useful biomarkers for diseases and cell differentiation states. sRNA-based biomarkers outperform traditional messenger RNA-based biomarkers by testing fewer targets with greater accuracy and providing earlier detection for disease states. Therefore, expression profiling of sRNAs is fundamentally important to further advance the understanding of biological processes, as well as diagnosis and treatment of diseases. High-throughput sequencing (HTS) is a powerful approach for both sRNA discovery and expression profiling. Here, we discuss the general considerations for sRNA-based HTS profiling methods from RNA preparation to sequencing library construction, with a focus on the causes of systematic error. By examining the enzymatic manipulation steps of sRNA expression profiling, this paper aims to demystify current HTS-based sRNA profiling approaches and to aid researchers in the informed design and interpretation of profiling experiments.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.