ArticleACS chemical neuroscience2010
Native serotonin membrane receptors recognize 5-hydroxytryptophan-functionalized substrates: enabling small-molecule recognition.
Article in ACS chemical neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 36 citations in OpenAlex.
- Serotonergic System-Targeted Nucleic Acid Hydrogel Coordinates Excitability Restoration and Circuit Reconstruction for Spinal Cord Injury Therapy.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Divalent Cation Dependence Enhances Dopamine Aptamer Biosensing.ACS applied materials & interfaces · 2021Article
- Enhancing Surface Capture and Sensing of Proteins with Low-Power Optothermal Bubbles in a Biphasic Liquid.Nano letters · 2020Article
- Aptamer Recognition of Multiplexed Small-Molecule-Functionalized Substrates.ACS applied materials & interfaces · 2018Article
- Small-Molecule Patterning via Prefunctionalized Alkanethiols.Chemistry of materials : a publication of the American Chemical Society · 2018Article
- Wafer-scale bioactive substrate patterning by chemical lift-off lithography.Beilstein journal of nanotechnology · 2018Article
- Neurochips Enable Nanoscale Devices for High-Resolution In Vivo Neurotransmitter Sensing.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2016Review
- Immobilization techniques for microarray: challenges and applications.Sensors (Basel, Switzerland) · 2014Review
- From the bottom up: dimensional control and characterization in molecular monolayers.Chemical Society reviews · 2013Review
- Nanotools for neuroscience and brain activity mapping.ACS nano · 2013Review
- Molecular plasmonics for biology and nanomedicine.Nanomedicine (London, England) · 2012Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recognition of small diffusible molecules by large biomolecules is ubiquitous in biology. To investigate these interactions, it is important to be able to immobilize small ligands on substrates; however, preserving recognition by biomolecule-binding partners under these circumstances is challenging. We have developed methods to modify substrates with serotonin, a small-molecule neurotransmitter important in brain function and psychiatric disorders. To mimic soluble serotonin, we attached its amino acid precursor, 5-hydroxytryptophan, via the ancillary carboxyl group to oligo(ethylene glycol)-terminated alkanethiols self-assembled on gold. Anti-5-hydroxytryptophan antibodies recognize these substrates, demonstrating bioavailability. Interestingly, 5-hydroxytryptophan-functionalized surfaces capture membrane-associated serotonin receptors enantiospecifically. By contrast, surfaces functionalized with serotonin itself fail to bind serotonin receptors. We infer that recognition by biomolecules evolved to distinguish small-molecule ligands in solution requires tethering of the latter via ectopic moieties. Membrane proteins, which are notoriously difficult to isolate, or other binding partners can be captured for identification, mapping, expression, and other purposes using this generalizable approach.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.