ArticleFood research international (Ottawa, Ont.)2012
Development and application of loop-mediated isothermal amplification assays on rapid detection of various types of staphylococci strains.
Article in Food research international (Ottawa, Ont.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Diagnosis of visceral and cutaneous leishmaniasis using loop-mediated isothermal amplification (LAMP) protocols: a systematic review and meta-analysis.Parasites & vectors · 2022Pooled it
- Hybridization chain reaction-based aptasensor for sensitive detection ofFood chemistry. Molecular sciences · 2026Article
- Genotypic and phenotypic strategies to detect antibiotic resistance in foodborne pathogens along the food processing chain.Current research in food science · 2026Review
- Rapid and visual detection of Shiga-toxigenic Escherichia coli (STEC) in carabeef meat harnessing loop-mediated isothermal amplification (LAMP).Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Development of a multiplex Loop-Mediated Isothermal Amplification (LAMP) for the diagnosis of bacterial periprosthetic joint infection.PloS one · 2024Article
- Antimicrobial Resistance, SCCAntibiotics (Basel, Switzerland) · 2023Article
- Genome mining reveals the prevalence and extensive diversity of toxin-antitoxin systems inFrontiers in microbiology · 2023Article
- Biofilm formation of two genetically diverseFrontiers in microbiology · 2023Article
- A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Feasibility of Loop-Mediated Isothermal Amplification for Rapid Detection of Methicillin-Susceptible and Methicillin-ResistantClinics in orthopedic surgery · 2022Article
- Formic acid, an organic acid food preservative, induces viable-but-non-culturable state, and triggers new Antimicrobial Resistance traits inFrontiers in microbiology · 2022Article
- Intense pulsed light for inactivating planktonic and biofilm molds in food.Frontiers in microbiology · 2022Article
- Quercetin modulates the gut microbiota as well as the metabolome in a rat model of osteoarthritis.Bioengineered · 2021Article
- The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis.Iranian journal of public health · 2021Review
- On-farm colorimetric detection of Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni in crude bovine nasal samples.Veterinary research · 2021Article
- One-Day Molecular Detection ofFoods (Basel, Switzerland) · 2021Article
- Microbiome characterization and re-design by biologic agents for inflammatory bowel disease insights.Bioprocess and biosystems engineering · 2021Review
- Characterization on gut microbiome of PCOS rats and its further design by shifts in high-fat diet and dihydrotestosterone induction in PCOS rats.Bioprocess and biosystems engineering · 2021Article
- Bioprocessing and integration of a high flux screening systematic platform based on isothermal amplification for the detection on 8 common pathogens.Bioprocess and biosystems engineering · 2021Article
- "One-step" characterization platform for pathogenic genetics of Staphylococcus aureus.Bioprocess and biosystems engineering · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
A loop-mediated isothermal amplification (LAMP) method for rapid detection of various Staphylococcus strains and associated antibiotic resistance determinant had been developed and evaluated in this study. Six primers, including outer primers, inner primers and loop primers, were specially designed for recognizing eight distinct sequences on three targets: 16SrRNA, femA and mecA.. Forty-one reference strains, including various species of gram-negative and -positive isolates, were included in this study to evaluate and optimize LAMP assays. The optimal reaction condition was found to be 65 °C for 45 min, with detection limits at 100 fg DNA/tube and 10 CFU/reaction for 16S rRNA, 100 fg DNA/tube and 10 CFU/reaction for femA, 1 pg DNA/tube and 100 CFU/reaction for mecA, respectively. Application of LAMP assays were performed on 118 various types of Staphylococcus isolates, the detection rate of LAMP assays for the 16SrRNA, femA and mecA was 100% (118/118), 98.5% (64/65) and 94.3% (66/70), and the negative predictive value (NPV) was 100%, 98.1% and 92.3% respectively; with a 100% positive predictive value (PPV) for all three targets. In conclusion, LAMP assays were demonstrated to be useful and powerful tools for rapid detection of various Staphylococcus strains, and undoubtedly, the rapidness, technical simplicity, and cost-effectiveness of LAMP assays will demonstrate broad application for bacteriological detection of food-borne Methicillin-resistant Staphylococcus (MRS) isolates.
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Registered trials
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