ArticleProceedings of the National Academy of Sciences of the United States of America2012
Hematopoietic-specific targeting of influenza A virus reveals replication requirements for induction of antiviral immune responses.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
63 citing papers in PubMed, 73 citations in OpenAlex.
- mRNA vaccine immunity is enhanced by hepatocyte detargeting and not dependent on dendritic cell expression.Nature biotechnology · 2026Article
- Optimal murine CD4Nature communications · 2026Article
- Limiting viral replication in hematopoietic cells delays Rift Valley fever virus disease progression in C57BL/6 mice.Journal of virology · 2025Article
- Lung cell fates during influenza.Cell research · 2025Review
- PB2 and NP of North American H5N1 virus drive immune cell replication and systemic infections.Science advances · 2025Article
- Review
- Tropism for ciliated cells is the dominant driver of influenza viral burst size in the human airway.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Segmented, Negative-Sense RNA Viruses of Humans: Genetic Systems and Experimental Uses of Reporter Strains.Annual review of virology · 2023Review
- Limiting viral replication in hepatocytes alters Rift Valley fever virus disease manifestations.Journal of virology · 2023Article
- Sex and molecular differences in cardiovascular parameters at peak influenza disease in mice.Physiological genomics · 2023Article
- A novel strategy to attenuate porcine reproductive and respiratory syndrome virus by inhibiting viral replication in the target pulmonary alveolar macrophages via hematopoietic-specific miR-142.One health advances · 2023Article
- Influenza virus replication in cardiomyocytes drives heart dysfunction and fibrosis.Science advances · 2022Article
- Characterization of influenza A virus induced transposons reveals a subgroup of transposons likely possessing the regulatory role as eRNAs.Scientific reports · 2022Article
- Mice with diverse microbial exposure histories as a model for preclinical vaccine testing.Cell host & microbe · 2021Article
- Lung dendritic cells migrate to the spleen to prime long-lived TCF1Science immunology · 2021Article
- Evaluation of Nutritional Gel Supplementation in C57BL/6J Mice Infected with Mouse-Adapted Influenza A/PR/8/34 Virus.Comparative medicine · 2020Article
- Synthetic Virology: Building Viruses to Better Understand Them.Cold Spring Harbor perspectives in medicine · 2020Review
- Ectromelia-encoded virulence factor C15 specifically inhibits antigen presentation to CD4+ T cells post peptide loading.PLoS pathogens · 2020Article
- Zika virus tropism during early infection of the testicular interstitium and its role in viral pathogenesis in the testes.PLoS pathogens · 2020Article
- MiRNA Targeted NP Genome of Live Attenuated Influenza Vaccines Provide Cross-Protection against a Lethal Influenza Virus Infection.Vaccines · 2020Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A coordinated innate and adaptive immune response, orchestrated by antigen presenting cells (APCs), is required for effective clearance of influenza A virus (IAV). Although IAV primarily infects epithelial cells of the upper respiratory tract, APCs are also susceptible. To determine if virus transcription in these cells is required to generate protective innate and adaptive immune responses, we engineered IAV to be selectively attenuated in cells of hematopoietic origin. Incorporation of hematopoietic-specific miR-142 target sites into the nucleoprotein of IAV effectively silenced virus transcription in APCs, but had no significant impact in lung epithelial cells. Here we demonstrate that inhibiting IAV replication in APCs in vivo did not alter clearance, or the generation of IAV-specific CD8 T cells, suggesting that cross-presentation is sufficient for cytotoxic T lymphocyte activation. In contrast, loss of in vivo virus infection, selectively in APCs, resulted in a significant reduction of retinoic acid-inducible gene I-dependent type I IFN (IFN-I). These data implicate the formation of virus replication intermediates in APCs as the predominant trigger of IFN-I in vivo. Taking these data together, this research describes a unique platform to study the host response to IAV and provides insights into the mechanism of antigen presentation and the induction of IFN-I.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.