ArticleProceedings of the National Academy of Sciences of the United States of America2012
Regulatory switch enforced by basic helix-loop-helix and ACT-domain mediated dimerizations of the maize transcription factor R.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed.
- Seed-specific overexpression of DREB2G alters seed morphology, oil accumulation, and germination in Arabidopsis.Plant cell reports · 2026Article
- Comparative transcriptomics of anthocyanin accumulation in the pericarp of pigmented purple corn.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Two mutations in the same MYC-bHLH transcription factor cause segregation of purple coloration of stolons and seed heads inHorticulture research · 2025Article
- The basic helix-loop-helix (bHLH) transcription factor DTT1 is part of a paired key that unlocks the tapetum transition in barley anther development.The Plant cell · 2025Article
- Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis.Plant physiology · 2025Article
- Regulation of Flavonoid Biosynthesis by the MYB-bHLH-WDR (MBW) Complex in Plants and Its Specific Features in Cereals.International journal of molecular sciences · 2025Review
- Proanthocyanidin biosynthesis and postharvest seed coat darkening in pinto bean.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2025Review
- A putative membrane-associated YdjB-like protein from Salmonella enterica exhibits a non-canonical ACT-like fold.PloS one · 2025Article
- Molecular insights into postharvest seed coat darkening in common beans: a look beyond theFrontiers in plant science · 2025Article
- CaMYBA-CaMYC-CaTTG1 complex activates the transcription of anthocyanin synthesis structural genes and regulates anthocyanin accumulation in pepper (Frontiers in plant science · 2025Article
- A single-nucleotide insertion in Rxp confers durable resistance to bacterial pustule in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Chemical inhibition of stomatal differentiation by perturbation of the master-regulatory bHLH heterodimer via an ACT-Like domain.Nature communications · 2024Article
- T and Z, partial seed coat patterning genes in common bean, provide insight into the structure and protein interactions of a plant MBW complex.G3 (Bethesda, Md.) · 2024Article
- Evolution and diversification of the ACT-like domain associated with plant basic helix-loop-helix transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- R3-MYB repressor Mybr97 is a candidate gene associated with the Anthocyanin3 locus and enhanced anthocyanin accumulation in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Rearrangement with the nkd2 promoter contributed to allelic diversity of the r1 gene in maize (Zea mays).The Plant journal : for cell and molecular biology · 2022Article
- Flavonoid Metabolic Profiles and Gene Mapping of Rice (Oryza sativa L.) Purple Gradient Grain Hulls.Rice (New York, N.Y.) · 2022Article
- Sunflower WRINKLED1 Plays a Key Role in Transcriptional Regulation of Oil Biosynthesis.International journal of molecular sciences · 2022Article
- Intragenic suppressors unravel the role of the SCREAM ACT-like domain for bHLH partner selectivity in stomatal development.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Functional Antagonism of WRI1 and TCP20 ModulatesPlants (Basel, Switzerland) · 2022Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The maize R2R3-MYB regulator C1 cooperates with the basic helix-loop-helix (bHLH) factor R to activate the expression of anthocyanin biosynthetic genes coordinately. As is the case for other bHLH factors, R harbors several protein-protein interaction domains. Here we show that not the classical but rather a briefly extended R bHLH region forms homodimers that bind canonical G-box DNA motifs. This bHLH DNA-binding activity is abolished if the C-terminal ACT (aspartokinase, chorismate, and TyrA) domain is licensed to homodimerize. Then the bHLH remains in the monomeric form, allowing it to interact with R-interacting factor 1 (RIF1). In this configuration, the R-RIF1 complex is recruited to the promoters of a subset of anthocyanin biosynthetic genes, such as A1, through the interaction with its MYB partner C1. If, however, the ACT domain remains monomeric, the bHLH region dimerizes and binds to G-boxes present in several anthocyanin genes, such as Bz1. Our results provide a mechanism by which a dimerization domain in a bHLH factor behaves as a switch that permits distinct configurations of a regulatory complex to be tethered to different promoters. Such a combinatorial gene regulatory framework provides one mechanism by which genes lacking obviously conserved cis-regulatory elements are regulated coordinately.
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