ArticleMolecular plant pathology2012
Subcellular targeting of an evolutionarily conserved plant defensin MtDef4.2 determines the outcome of plant-pathogen interaction in transgenic Arabidopsis.
Article in Molecular plant pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 34 citations in OpenAlex.
- Genomic and functional insights into the diversity of3 Biotech · 2025Article
- Research Progress and Prospect of Alfalfa Resistance to Pathogens and Pests.Plants (Basel, Switzerland) · 2022Review
- Use of Defensins to Develop Eco-Friendly Alternatives to Synthetic Fungicides to Control Phytopathogenic Fungi and Their Mycotoxins.Journal of fungi (Basel, Switzerland) · 2022Review
- Time Series Transcriptome Analysis inFrontiers in plant science · 2022Article
- Antifungal Peptides and Proteins to Control Toxigenic Fungi and Mycotoxin Biosynthesis.International journal of molecular sciences · 2021Review
- Peanuts that keep aflatoxin at bay: a threshold that matters.Plant biotechnology journal · 2018Article
- Expression of apoplast-targeted plant defensin MtDef4.2 confers resistance to leaf rust pathogen Puccinia triticina but does not affect mycorrhizal symbiosis in transgenic wheat.Transgenic research · 2017Article
- Nicotiana alata Defensin Chimeras Reveal Differences in the Mechanism of Fungal and Tumor Cell Killing and an Enhanced Antifungal Variant.Antimicrobial agents and chemotherapy · 2016Article
- Functional characterization of Rorippa indica defensin and its efficacy against Lipaphis erysimi.SpringerPlus · 2016Article
- Mutations in the Arabidopsis homoserine kinase gene DMR1 confer enhanced resistance to Fusarium culmorum and F. graminearum.BMC plant biology · 2014Article
- Insect antimicrobial peptides and their applications.Applied microbiology and biotechnology · 2014Review
- Field resistance to Fusarium oxysporum and Verticillium dahliae in transgenic cotton expressing the plant defensin NaD1.Journal of experimental botany · 2014Article
- Inhibition of cereal rust fungi by both class I and II defensins derived from the flowers of Nicotiana alata.Molecular plant pathology · 2014Article
- Spatio-temporal expression patterns of Arabidopsis thaliana and Medicago truncatula defensin-like genes.PloS one · 2013Article
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9 authors at 4 institutions in 2 countries.
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Abstract
The Medicago truncatula gene encoding an evolutionarily conserved antifungal defensin MtDef4.2 was cloned and characterized. In silico expression analysis indicated that MtDef4.2 is expressed in many tissues during the normal growth and development of M. truncatula. MtDef4.2 exhibits potent broad-spectrum antifungal activity against various Fusarium spp. Transgenic Arabidopsis thaliana lines in which MtDef4.2 was targeted to three different subcellular compartments were generated. These lines were tested for resistance to the obligate biotrophic oomycete Hyaloperonospora arabidopsidis Noco2 and the hemibiotrophic fungal pathogen Fusarium graminearum PH-1. MtDef4.2 directed to the extracellular space, but not to the vacuole or retained in the endoplasmic reticulum, conferred robust resistance to H. arabidopsidis. Siliques of transgenic Arabidopsis lines expressing either extracellularly or intracellularly targeted MtDef4.2 displayed low levels of resistance to F. graminearum, but accumulated substantially reduced levels of the mycotoxin deoxynivalenol. The data presented here suggest that extracellularly targeted MtDef4.2 is sufficient to provide strong resistance to the biotrophic oomycete, consistent with the extracellular lifestyle of this pathogen. However, the co-expression of extracellular and intracellular MtDef4.2 is probably required to achieve strong resistance to the hemibiotrophic pathogen F. graminearum which grows extracellularly and intracellularly.
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