ArticleJournal of biological engineering2012
A genetic bistable switch utilizing nonlinear protein degradation.
Article in Journal of biological engineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- T7 phage-assisted evolution of riboswitches using error-prone replication and dual selection.Scientific reports · 2024Article
- Review
- Article
- Modelling co-translational dimerization for programmable nonlinearity in synthetic biology.Journal of the Royal Society, Interface · 2020Article
- Accelerated evolution of a minimal 63-amino acid dual transcription factor.Science advances · 2020Article
- Translating New Synthetic Biology Advances for Biosensing Into the Earth and Environmental Sciences.Frontiers in microbiology · 2020Review
- Synchrony and pattern formation of coupled genetic oscillators on a chip of artificial cells.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Engineering orthogonal dual transcription factors for multi-input synthetic promoters.Nature communications · 2016Article
- A computational method for the investigation of multistable systems and its application to genetic switches.BMC systems biology · 2016Article
- Genetic switches and related tools for controlling gene expression and electrical outputs of Geobacter sulfurreducens.Journal of industrial microbiology & biotechnology · 2016Article
- The limiting dynamics of a bistable molecular switch with and without noise.Journal of mathematical biology · 2016Article
- A transcription activator-like effector (TALE) induction system mediated by proteolysis.Nature chemical biology · 2016Article
- Article
- Robust, tunable genetic memory from protein sequestration combined with positive feedback.Nucleic acids research · 2015Article
- Tunable protein degradation in bacteria.Nature biotechnology · 2014Article
- Gene networks of fully connected triads with complete auto-activation enable multistability and stepwise stochastic transitions.PloS one · 2014Article
- Tailored fatty acid synthesis via dynamic control of fatty acid elongation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBistability is a fundamental property in engineered and natural systems, conferring the ability to switch and retain states. Synthetic bistable switches in prokaryotes have mainly utilized transcriptional components in their construction. Using both transcriptional and enzymatic components, creating a hybrid system, allows for wider bistable parameter ranges in a circuit.
resultsIn this paper, we demonstrate a tunable family of hybrid bistable switches in E. coli using both transcriptional components and an enzymatic component. The design contains two linked positive feedback loops. The first loop utilizes the lambda repressor, CI, and the second positive feedback loop incorporates the Lon protease found in Mesoplasma florum (mf-Lon). We experimentally tested for bistable behavior in exponential growth phase, and found that our hybrid bistable switch was able to retain its state in the absence of an input signal throughout 40 cycles of cell division. We also tested the transient behavior of our switch and found that switching speeds can be tuned by changing the expression rate of mf-Lon.
conclusionsTo our knowledge, this work demonstrates the first use of dynamic expression of an orthogonal and heterologous protease to tune a nonlinear protein degradation circuit. The hybrid switch is potentially a more robust and tunable topology for use in prokaryotic systems.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.