Trial reportJournal of the American Association for Laboratory Animal Science : JAALAS2012
Effects of buprenorphine on a cecal ligation and puncture model in C57BL/6 mice.
Trial report in Journal of the American Association for Laboratory Animal Science : JAALAS, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 39 citations in OpenAlex.
- Benefits of 21% Oxygen Compared with 100% Oxygen for Delivery of Isoflurane to Mice (Journal of the American Association for Laboratory Animal Science : JAALAS · 2017Trial
- Experimental robustness and reproducibility of the murine cecal ligation and puncture sepsis model.Intensive care medicine experimental · 2026Article
- Buprenorphine extended-release (Ethiqa XR) impacts the immunological response in mice exposed to aerosolizedFrontiers in immunology · 2026Article
- Repurposing a Detrimental Antibody Epitope as Targeted Therapeutics for Sepsis and Rheumatoid Arthritis.Research square · 2025Article
- The ACTH test fails to diagnose adrenal insufficiency and augments cytokine production in sepsis.JCI insight · 2025Article
- Pro-dermcidin and derivatives as potential therapeutics for lethal experimental sepsis.Frontiers in immunology · 2025Article
- Repurposing a detrimental antibody epitope as targeted therapeutics for sepsis and rheumatoid arthritis.Military Medical Research · 2025Article
- Therapeutic potential of procathepsin L-inhibiting and progesterone-entrapping dimethyl-β-cyclodextrin nanoparticles in treating experimental sepsis.Frontiers in immunology · 2024Article
- Common Variables That Influence Sepsis Mortality in Mice.Journal of inflammation research · 2023Article
- Anesthesia and analgesia for common research models of adult mice.Laboratory animal research · 2022Review
- A Review of Long-acting Parenteral Analgesics for Mice and Rats.Journal of the American Association for Laboratory Animal Science : JAALAS · 2022Review
- The Effects of Biological Sex on Sepsis Treatments in Animal Models: A Systematic Review and a Narrative Elaboration on Sex- and Gender-Dependent Differences in Sepsis.Critical care explorations · 2021Review
- Analgesia and Humane Endpoints for Rodents in Sepsis Research.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Inducing Experimental Polymicrobial Sepsis by Cecal Ligation and Puncture.Current protocols in immunology · 2020Article
- Kinin B1 receptor: a potential therapeutic target in sepsis-induced vascular hyperpermeability.Journal of translational medicine · 2020Article
- The Systemic and Cellular Metabolic Phenotype of Infection and Immune Response toFrontiers in immunology · 2020Article
- The Influence of Pain and Analgesia in Rodent Models of Sepsis.Comparative medicine · 2019Review
- Buprenorphine Markedly Elevates a Panel of Surrogate Markers in a Murine Model of Sepsis.Shock (Augusta, Ga.) · 2019Article
- Part I: Minimum Quality Threshold in Preclinical Sepsis Studies (MQTiPSS) for Study Design and Humane Modeling Endpoints.Shock (Augusta, Ga.) · 2019Review
- To Treat or Not to Treat: The Effects of Pain on Experimental Parameters.Comparative medicine · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sepsis research relies heavily on animal models. One of the most frequently used models, cecal ligation and puncture (CLP), involves surgery, and animal use committees may require the use of analgesics after CLP. However, some analgesics are immunomodulatory and may affect research outcomes. In addition, both septic inflammation and responses to opioids may vary with the sex of the subject. Therefore, we investigated the effects of buprenorphine in inbred mice of both sexes undergoing CLP. We hypothesized that buprenorphine would not significantly change the outcome or patterns of inflammation in C57BL/6 mice after CLP. Male and female C57BL/6 mice underwent CLP surgery and were randomized into 2 groups to receive either buprenorphine or saline. Three-week survival studies were performed (n = 20 per group). Survival did not differ between groups of female mice, but male mice that received buprenorphine had decreased survival compared with that of controls. Reducing the dose of buprenorphine in male mice ameliorated the difference in survival. To examine inflammation, mice (n = 10 per group) were euthanized at 12, 24, or 48 h after CLP. Cell counts and cytokines were measured in the blood and peritoneal lavage fluid. In female and male C57BL/6 mice, buprenorphine treatment resulted in few differences in inflammatory parameters, although peripheral neutrophil counts were decreased transiently in male mice. The findings suggest that the effects of buprenorphine on sepsis models in C57BL/6 mice may be sex-specific. Consequently the use of analgesics must be assessed on a study-by-study basis, and investigators should define analgesic regimens when publishing sepsis studies.
Indexed as
Identifiers
22776195PMC3358986W1953293555What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.