ArticleScientific reports2012
Intersectional Cre driver lines generated using split-intein mediated split-Cre reconstitution.
Article in Scientific reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli.Journal of biological engineering · 2025Article
- Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy.Frontiers in bioengineering and biotechnology · 2024Article
- Review
- Neural circuit-specific gene manipulation in mouse brainSTAR protocols · 2022Article
- The whisking oscillator circuit.Nature · 2022Article
- Article
- An intein-split transactivator for intersectional neural imaging and optogenetic manipulation.Nature communications · 2022Article
- Article
- Application of AAV1 for Anterograde Transsynaptic Circuit Mapping and Input-Dependent Neuronal Cataloging.Current protocols · 2022Article
- Recent advances in lineage tracing for the kidney.Kidney international · 2021Review
- Article
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- Intein-mediated cytoplasmic reconstitution of a split toxin enables selective cell ablation in mixed populations and tumor xenografts.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- RecV recombinase system for in vivo targeted optogenomic modifications of single cells or cell populations.Nature methods · 2020Article
- The application of gene splitting technique for controlling transgene flow in rice.Transgenic research · 2020Article
- Control of ϕC31 integrase-mediated site-specific recombination by protein trans-splicing.Nucleic acids research · 2019Article
- Bronchioalveolar stem cells are a main source for regeneration of distal lung epitheliaThe EMBO journal · 2019Article
- Biotechnological Applications of Protein Splicing.Current protein & peptide science · 2019Review
- Neural lineage tracing in the mammalian brain.Current opinion in neurobiology · 2018Review
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Authors and funding
7 authors.
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Abstract
Tissue and cell type highly specific Cre drivers are very rare due to the fact that most genes or promoters used to direct Cre expressions are generally expressed in more than one tissues and/or in multiple cell types. We developed a split-intein based split-Cre system for highly efficient Cre-reconstitution through protein splicing. This split-intein-split-Cre system can be used to intersect the expression patterns of two genes or promoters to restrict full-length Cre reconstitution in their overlapping domains. To test this system in vivo, we selected several conserved human enhancers to drive the expression of either Cre-N-intein-N, or intein-C-Cre-C transgene in different brain regions. In all paired CreN/CreC transgenic mice, Cre-dependent reporter was efficiently induced specifically in the intersectional expression domains of two enhancers. This split-intein based method is simpler to implement compared with other strategies for generating highly-restricted intersectional Cre drivers to study complex tissues such as the nervous system.
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