Evidence map›Paper›PMID 22773833›Full record

ArticleThe Journal of biological chemistry2012

Diindolylmethane-mediated Gli1 protein suppression induces anoikis in ovarian cancer cells in vitro and blocks tumor formation ability in vivo.

Prabodh K Kandala, Sanjay K Srivastava

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 44 citations in OpenAlex.

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  9. Phytochemicals in Gynecological Cancer Prevention.International journal of molecular sciences · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Prabodh K KandalaDepartment of Biomedical Sciences, Texas Tech University Health Sciences Center, Amarillo, Texas 79106, USA.
Sanjay K Srivastava
Texas Tech University · US

Funding

Chemoprevention of Pancreatic Cancer by CapsaicinR01CA129038 · NCI · TEXAS TECH UNIVERSITY HEALTH SCIS CENTER · PI SRIVASTAVA, SANJAY K. · 2009 to 2013
$1.5M
Role of Nuclear Factor kappa Beta in Activity of BITC against Pancreatic CancerR01CA106953 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SRIVASTAVA, SANJAY K. · 2004 to 2008
$1.5M
NCI NIH HHS CA129038NCI NIH HHS R01 CA106953NCI NIH HHS R01 CA129038
6 · The paper itself

Abstract

Anoikis is a cell death that occurs due to detachment of a cell from the extracellular matrix (ECM). Resistance to anoikis is a primary feature of a cell that undergoes metastasis. In this study for the first time, we demonstrated the potential role of Gli1 in anoikis resistance. Treatment of various ovarian cancer cells by different concentrations of diindolylmethane (DIM), an active ingredient of cruciferous vegetables, reduced the anoikis resistance in a concentration-dependent manner. Reduction in anoikis resistance was associated with a decrease in the expression of Gli1 and an increase in the cleavage of poly(ADP-ribose) polymerase (PARP). Sonic hedgehog (Shh) treatment not only increased the expression of Gli1, but also blocked anoikis induced by DIM and abrogated the change in the expression of Gli1 and cleaved PARP by DIM. To confirm the role of Gli1, hedgehog inhibitor cyclopamine, Gli1 siRNA and Gli1(-/-) mouse embryonic fibroblasts (MEFs) were used. Cyclopamine treatment alone significantly reduced anoikis resistance in A2780 and OVCAR-429 cells. Cyclopamine-mediated reduction in anoikis resistance was associated with reduced expression of Gli1 and induction of cleaved PARP. Shh treatment blocked cyclopamine-induced anoikis. Silencing Gli1 expression induced anoikis and cleavage of PARP in A2780 and OVCAR-429 cells. Furthermore, Gli1(-/-) MEFs were more sensitive to anoikis compared with Gli1(+/+) MEFs. Our in vivo studies established that DIM- or cyclopamine-treated ovarian cancer cells under suspension culture conditions drastically lost their ability of tumor formation in vivo in mice. Taken together, our results establish that Gli1 is a critical player in anoikis resistance in ovarian cancer.

Indexed as

AnimalsAnoikisCell Line, TumorDrug Resistance, NeoplasmEmbryo, MammalianFemaleFibroblastsGene Expression Regulation, NeoplasticHedgehog ProteinsIndolesKruppel-Like Transcription FactorsMiceMice, KnockoutOvarian NeoplasmsPoly(ADP-ribose) PolymerasesVeratrum Alkaloids3,3'-diindolylmethanecyclopamineGli1 protein, mouseHedgehog ProteinsIndolesKruppel-Like Transcription FactorsPoly(ADP-ribose) PolymerasesShh protein, mouseVeratrum AlkaloidsZinc Finger Protein GLI1

Identifiers

PMID22773833
PMCPMC3436512
OpenAlexW2042753965

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.