ArticleJournal of bacteriology2012
Nucleotides critical for the interaction of the Streptococcus pyogenes Mga virulence regulator with Mga-regulated promoter sequences.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- The global transcriptional regulator MgaSpn affects the virulence of Streptococcus pneumoniae by regulating PcpA.BMC microbiology · 2025Article
- Molecular Targets inGenes · 2024Review
- One Earth: The Equilibrium between the Human and the Bacterial Worlds.International journal of molecular sciences · 2023Review
- Promoter DNA recognition by theFrontiers in molecular biosciences · 2023Article
- Functional and Transcriptome Analysis ofInternational journal of molecular sciences · 2022Article
- MgaVirulence · 2021Article
- PRD-Containing Virulence Regulators (PCVRs) in Pathogenic Bacteria.Frontiers in cellular and infection microbiology · 2021Review
- Phosphotransferase System Uptake and Metabolism of the β-Glucoside Salicin Impact Group A Streptococcal Bloodstream Survival and Soft Tissue Infection.Infection and immunity · 2020Article
- Transcriptional activation by MafR, a global regulator of Enterococcus faecalis.Scientific reports · 2019Article
- DNA-binding properties of MafR, a global regulator of Enterococcus faecalis.FEBS letters · 2018Article
- Genomic Comparison among Lethal Invasive Strains ofFrontiers in microbiology · 2017Article
- MgaFrontiers in molecular biosciences · 2016Article
- Phosphorylation events in the multiple gene regulator of group A Streptococcus significantly influence global gene expression and virulence.Infection and immunity · 2015Article
- Global Regulation of Gene Expression by the MafR Protein of Enterococcus faecalis.Frontiers in microbiology · 2015Article
- Article
- Clinical laboratory response to a mock outbreak of invasive bacterial infections: a preparedness study.Journal of clinical microbiology · 2014Article
- The pneumococcal MgaSpn virulence transcriptional regulator generates multimeric complexes on linear double-stranded DNA.Nucleic acids research · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Mga regulator of Streptococcus pyogenes directly activates the transcription of a core regulon that encodes virulence factors such as M protein (emm), C5a peptidase (scpA), and streptococcal inhibitor of complement (sic) by directly binding to a 45-bp binding site as determined by an electrophoretic mobility shift assay (EMSA) and DNase I protection. However, by comparing the nucleotide sequences of all established Mga binding sites, we found that they exhibit only 13.4% identity with no discernible symmetry. To determine the core nucleotides involved in functional Mga-DNA interactions, the M1T1 Pemm1 binding site was altered and screened for nucleotides important for DNA binding in vitro and for transcriptional activation using a plasmid-based luciferase reporter in vivo. Following this analysis, 34 nucleotides within the Pemm1 binding site that had an effect on Mga binding, Mga-dependent transcriptional activation, or both were identified. Of these critical nucleotides, guanines and cytosines within the major groove were disproportionately identified clustered at the 5' and 3' ends of the binding site and with runs of nonessential adenines between the critical nucleotides. On the basis of these results, a Pemm1 minimal binding site of 35 bp bound Mga at a level comparable to the level of binding of the larger 45-bp site. Comparison of Pemm with directed mutagenesis performed in the M1T1 Mga-regulated PscpA and Psic promoters, as well as methylation interference analysis of PscpA, establish that Mga binds to DNA in a promoter-specific manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.