ArticleThe Plant cell2012
Reconstitution of plant alkane biosynthesis in yeast demonstrates that Arabidopsis ECERIFERUM1 and ECERIFERUM3 are core components of a very-long-chain alkane synthesis complex.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 201 papers.
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Who cites it
201 citing papers in PubMed, 477 citations in OpenAlex.
- Review
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- The algal homolog of the plant CER1 and CER3 proteins is a bifunctional hydrocarbon-forming enzyme.The New phytologist · 2026Article
- Wheat MYB46-like Transcription Factor Stimulates Cuticular Wax Biosynthesis.Biomolecules · 2026Article
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- Laminar patterning transcription factors orchestrate spatial metabolite partitioning in Capsicum fruit.Nature plants · 2026Article
- ABC transporter BrABCG12 mutation results in tender green glossy leaves in Chinese cabbage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Comparative Metabolomics Reveals Family-Genus-Specific Chemical Signatures and Potential Recognition Mechanisms inMolecules (Basel, Switzerland) · 2026Article
- Wheat Class I TCP Transcription Factor TaTCP15 Positively Regulates Cutin and Cuticular Wax Biosynthesis.Biomolecules · 2026Article
- Comparative Genomic Analysis and Functional Identification of CER1 and CER3 Homologs in Rice Wax Synthesis.Biology · 2026Article
- Structural basis of VLCFA chain-length determination by the KCS6-CER2 complex in plants.Science advances · 2025Article
- Blue-light-enhanced interaction between ZmCRY1s and GL2 modulates epidermal wax composition in Zea mays.Nature communications · 2025Article
- Identification of a Leaf Cuticular Wax Biosynthesis GenePlants (Basel, Switzerland) · 2025Article
- Taxonomic distribution of alkane synthesizing aldehyde dehydrogenases across bacteria, fungi, plants, and animals.Biotechnology for biofuels and bioproducts · 2025Article
- Elucidating the drought-responsive changes in poplar cuticular waxes: a GWAS analysis of genes involved in fatty acid biosynthesis.Tree physiology · 2025Article
- BrCER1 intron mutation causing a wax deficient phenotype in Chinese cabbage.BMC plant biology · 2025Article
- Mutations in BrMYB31 lead to a glossy phenotype caused by a deficiency in epidermal wax crystals in Chinese cabbage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Directed evolution of hydrocarbon-producing enzymes.Biotechnology for biofuels and bioproducts · 2025Review
- Metabolic and genetic analysis links TRITERPENE SYNTHASE 12 to oleanolic acid biosynthesis in grape berry wax.Journal of experimental botany · 2025Article
- Insights into the Molecular Basis of Pollen Coat Development and Its Role in Male Sterility.International journal of molecular sciences · 2025Review
141 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In land plants, very-long-chain (VLC) alkanes are major components of cuticular waxes that cover aerial organs, mainly acting as a waterproof barrier to prevent nonstomatal water loss. Although thoroughly investigated, plant alkane synthesis remains largely undiscovered. The Arabidopsis thaliana ECERIFERUM1 (CER1) protein has been recognized as an essential element of wax alkane synthesis; nevertheless, its function remains elusive. In this study, a screen for CER1 physical interaction partners was performed. The screen revealed that CER1 interacts with the wax-associated protein ECERIFERUM3 (CER3) and endoplasmic reticulum-localized cytochrome b5 isoforms (CYTB5s). The functional relevance of these interactions was assayed through an iterative approach using yeast as a heterologous expression system. In a yeast strain manipulated to produce VLC acyl-CoAs, a strict CER1 and CER3 coexpression resulted in VLC alkane synthesis. The additional presence of CYTB5s was found to enhance CER1/CER3 alkane production. Site-directed mutagenesis showed that CER1 His clusters are essential for alkane synthesis, whereas those of CER3 are not, suggesting that CYTB5s are specific CER1 cofactors. Collectively, our study reports the identification of plant alkane synthesis enzymatic components and supports a new model for alkane production in which CER1 interacts with both CER3 and CYTB5 to catalyze the redox-dependent synthesis of VLC alkanes from VLC acyl-CoAs.
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