ArticleApplied and environmental microbiology2012
Characterization of a novel microcin that kills enterohemorrhagic Escherichia coli O157:H7 and O26.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Semantic search using protein large language models detects class II microcins in bacterial genomes.mSystems · 2024Article
- Class IIb Microcin MccM Interferes with Oxidative Phosphorylation inACS chemical biology · 2024Article
- Semantic search using protein large language models detects class II microcins in bacterial genomes.bioRxiv : the preprint server for biology · 2023Article
- Evidence for Widespread Class II Microcins inApplied and environmental microbiology · 2022Article
- Microcins reveal natural mechanisms of bacterial manipulation to inform therapeutic development.Microbiology (Reading, England) · 2022Article
- Article
- Potential Novel Food-Related and Biomedical Applications of Nanomaterials Combined with Bacteriocins.Pharmaceutics · 2021Review
- A Toxic Environment: a Growing Understanding of How Microbial Communities Affect Escherichia coli O157:H7 Shiga Toxin Expression.Applied and environmental microbiology · 2020Review
- Bacteriocins to Thwart Bacterial Resistance in Gram Negative Bacteria.Frontiers in microbiology · 2020Review
- A Putative Microcin Amplifies Shiga Toxin 2a Production of Escherichia coli O157:H7.Journal of bacteriology · 2019Article
- Microcin PDI Inhibits Antibiotic-Resistant Strains of Escherichia coli andApplied and environmental microbiology · 2019Article
- Optimisation of the Protocol for the LIVE/DEADFrontiers in microbiology · 2019Article
- Microcins inFrontiers in microbiology · 2019Review
- Modulation of EnterohaemorrhagicMicroorganisms · 2018Review
- Microcin PDI regulation and proteolytic cleavage are unique among known microcins.Scientific reports · 2017Article
- Autoinducer-2 Quorum Sensing Contributes to Regulation of Microcin PDI inFrontiers in microbiology · 2017Article
- Whole-Genome Sequencing Identifies In Vivo Acquisition of a blaCTX-M-27-Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection.Antimicrobial agents and chemotherapy · 2016Article
- Genome Sequence of Bibersteinia trehalosi Strain Y31 Isolated from the Pneumonic Lung of a Bighorn Sheep.Genome announcements · 2016Article
- Protozoan Predation of Escherichia coli O157:H7 Is Unaffected by the Carriage of Shiga Toxin-Encoding Bacteriophages.PloS one · 2016Article
- Genome-Wide Screening Identifies Six Genes That Are Associated with Susceptibility to Escherichia coli Microcin PDI.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel phenotype was recently identified in which specific strains of Escherichia coli inhibit competing E. coli strains via a mechanism that was designated "proximity-dependent inhibition" (PDI). PDI-expressing (PDI(+)) E. coli is known to inhibit susceptible (PDI(-)) E. coli strains, including several enterohemorrhagic (EHEC) and enterotoxigenic (ETEC) E. coli strains. In this study, every strain from a genetically diverse panel of E. coli O157:H7 (n = 25) and additional strains of E. coli serovar O26 were susceptible to the PDI phenotype. LIVE/DEAD staining was consistent with inhibition by killing of susceptible cells. Comparative genome analysis identified the genetic component of PDI, which is composed of a plasmid-borne (Incl1) operon encoding a putative microcin and associated genes for transport, immunity, and microcin activation. Transfer of the plasmid to a PDI(-) strain resulted in transfer of the phenotype, and deletion of the genes within the operon resulted in loss of the inhibition phenotype. Deletion of chromosomally encoded tolC also resulted in loss of the inhibitory phenotype, and this confirmed that the putative microcin is most likely secreted via a type I secretion pathway. Deletion of an unrelated plasmid gene did not affect the PDI phenotype. Quantitative reverse transcription (RT)-PCR demonstrated that microcin expression is correlated with logarithmic-phase growth. The ability to inhibit a diversity of E. coli strains indicates that this microcin may influence gut community composition and could be useful for control of important enteric pathogens.
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