ArticleApplied and environmental microbiology2012
A convenient and robust in vivo reporter system to monitor gene expression in the human pathogen Helicobacter pylori.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach.Nature communications · 2023Article
- TheFrontiers in microbiology · 2018Article
- Metal-responsive promoter DNA compaction by the ferric uptake regulator.Nature communications · 2016Article
- The cag-pathogenicity island encoded CncR1 sRNA oppositely modulates Helicobacter pylori motility and adhesion to host cells.Cellular and molecular life sciences : CMLS · 2016Article
- An enhanced chimeric firefly luciferase-inspired enzyme for ATP detection and bioluminescence reporter and imaging applications.Analytical biochemistry · 2015Article
- An easy way for the rapid purification of recombinant proteins from Helicobacter pylori using a newly designed expression vector.Journal of microbiology (Seoul, Korea) · 2014Article
- Essential role for the response regulator PmrA in Coxiella burnetii type 4B secretion and colonization of mammalian host cells.Journal of bacteriology · 2014Article
- In depth analysis of the Helicobacter pylori cag pathogenicity island transcriptional responses.PloS one · 2014Article
- Control of gene expression in Helicobacter pylori using the Tet repressor.Journal of microbiological methods · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thirty years of intensive research have significantly contributed to our understanding of Helicobacter pylori biology and pathogenesis. However, the lack of convenient genetic tools, in particular the limited effectiveness of available reporter systems, has notably limited the toolbox for fundamental and applied studies. Here, we report the construction of a bioluminescent H. pylori reporter system based on the Photorhabdus luminescens luxCDABE cassette. The system is constituted of a promoterless lux acceptor strain in which promoters and sequences of interest can be conveniently introduced by double homologous recombination of a suicide transformation vector. We validate the robustness of this new lux reporter system in noninvasive in vivo monitoring of dynamic transcriptional responses of inducible as well as repressible promoters and demonstrate its suitability for the implementation of genetic screens in H. pylori.
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Registered trials
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