ArticleApplied and environmental microbiology2012
Fast and sensitive detection of Bacillus anthracis spores by immunoassay.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 33 citations in OpenAlex.
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- Ultra-Sensitive Detection of Bacterial Spores via SERS.ACS sensors · 2025Article
- Review
- Dual-Readout Sandwich Immunoassay for Device-Free and Highly Sensitive Anthrax Biomarker Detection.Analytical chemistry · 2020Article
- Portable Rice Disease Spores Capture and Detection Method Using Diffraction Fingerprints on Microfluidic Chip.Micromachines · 2019Article
- Genetic Fusion of an Anti-BclA Single-Domain Antibody with Beta Galactosidase.Antibodies (Basel, Switzerland) · 2018Article
- Rapid Detection of Bacillus anthracis Spores Using Immunomagnetic Separation and Amperometry.Biosensors · 2016Article
- Identification and validation of specific markers of Bacillus anthracis spores by proteomics and genomics approaches.Molecular & cellular proteomics : MCP · 2014Article
- Quantitative and sensitive RNA based detection of Bacillus spores.Frontiers in microbiology · 2014Article
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus anthracis is one of the most dangerous potential biological weapons, and it is essential to develop a rapid and simple method to detect B. anthracis spores in environmental samples. The immunoassay is a rapid and easy-to-use method for the detection of B. anthracis by means of antibodies directed against surface spore antigens. With this objective in view, we have produced a panel of monoclonal antibodies against B. anthracis and developed colorimetric and electrochemiluminescence (ECL) immunoassays. Using Meso Scale Discovery ECL technology, which is based on electrochemiluminescence (ECL) detection utilizing a sulfo-Tag label that emits light upon electrochemical stimulation (using a dedicated ECL plate reader, an electrical current is placed across the microplate with electrodes integrated into the bottom of the plate, resulting in a series of electrically induced reactions leading to a luminescent signal), a detection limit ranging between 0.3 × 10(3) and 10(3) CFU/ml (i.e., 30 to 100 spores per test), depending on the B. anthracis strain assayed, was achieved. In complex matrices (5 mg/ml of soil or simulated powder), the detection level (without any sample purification or concentration) was never altered more than 3-fold compared with the results obtained in phosphate-buffered saline.
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Registered trials
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