ArticleExperimental & applied acarology2013
Gene silencing in tick cell lines using small interfering or long double-stranded RNA.
Article in Experimental & applied acarology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- SFTSV NSs protein is a tick antiviral RNAi response suppressor.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Gene silencing in the aedine cell lines C6/36 and U4.4 using long double-stranded RNA.Parasites & vectors · 2024Article
- The role of autophagy in tick-endosymbiont interactions: insights fromMicrobiology spectrum · 2024Article
- Nanoparticle-Shielded dsRNA Delivery for Enhancing RNAi Efficiency in Cotton Spotted BollwormInternational journal of molecular sciences · 2023Article
- Cationic Glycopolyelectrolytes for RNA Interference in Tick Cells.Biomacromolecules · 2022Article
- Article
- Identification and Characterization of Immunodominant Proteins from Tick Tissue Extracts Inducing a Protective Immune Response againstVaccines · 2021Article
- Article
- The Tick Cell Biobank: A global resource for in vitro research on ticks, other arthropods and the pathogens they transmit.Ticks and tick-borne diseases · 2018Review
- The Use of Ex Vivo Organ Cultures in Tick-Borne Virus Research.ACS infectious diseases · 2018Review
- Flavivirus Infection ofmBio · 2017Article
- RNAi reveals proteins for metabolism and protein processing associated with Langat virus infection in Ixodes scapularis (black-legged tick) ISE6 cells.Parasites & vectors · 2017Article
- Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.Scientific reports · 2016Article
- Microscopic Visualisation of Zoonotic Arbovirus Replication in Tick Cell and Organ Cultures Using Semliki Forest Virus Reporter Systems.Veterinary sciences · 2016Article
- Transmission bottlenecks and RNAi collectively influence tick-borne flavivirus evolution.Virus evolution · 2016Article
- Ixodes scapularis and Ixodes ricinus tick cell lines respond to infection with tick-borne encephalitis virus: transcriptomic and proteomic analysis.Parasites & vectors · 2015Article
- Review
- Article
- Induction and suppression of tick cell antiviral RNAi responses by tick-borne flaviviruses.Nucleic acids research · 2014Article
- Coinfection of tick cell lines has variable effects on replication of intracellular bacterial and viral pathogens.Ticks and tick-borne diseases · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Gene silencing by RNA interference (RNAi) is an important research tool in many areas of biology. To effectively harness the power of this technique in order to explore tick functional genomics and tick-microorganism interactions, optimised parameters for RNAi-mediated gene silencing in tick cells need to be established. Ten cell lines from four economically important ixodid tick genera (Amblyomma, Hyalomma, Ixodes and Rhipicephalus including the sub-species Boophilus) were used to examine key parameters including small interfering RNA (siRNA), double stranded RNA (dsRNA), transfection reagent and incubation time for silencing virus reporter and endogenous tick genes. Transfection reagents were essential for the uptake of siRNA whereas long dsRNA alone was taken up by most tick cell lines. Significant virus reporter protein knockdown was achieved using either siRNA or dsRNA in all the cell lines tested. Optimum conditions varied according to the cell line. Consistency between replicates and duration of incubation with dsRNA were addressed for two Ixodes scapularis cell lines; IDE8 supported more consistent and effective silencing of the endogenous gene subolesin than ISE6, and highly significant knockdown of the endogenous gene 2I1F6 in IDE8 cells was achieved within 48 h incubation with dsRNA. In summary, this study shows that gene silencing by RNAi in tick cell lines is generally more efficient with dsRNA than with siRNA but results vary between cell lines and optimal parameters need to be determined for each experimental system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.