ArticleIntegrative biology : quantitative biosciences from nano to macro2012
Functional characterization and axonal transport of quantum dot labeled BDNF.
Article in Integrative biology : quantitative biosciences from nano to macro, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Huntingtin polyglutamine expansions misdirect axonal transport by perturbing motor and adaptor recruitment.iScience · 2026Article
- LF-DBS of the ventral striatum shortens persistence for morphine place preference and modulates BDNF expression in the hippocampus.Behavioural brain research · 2025Article
- PLC-γ-CaFrontiers in molecular neuroscience · 2024Article
- Trifunctional Saxitoxin Conjugates for Covalent Labeling of Voltage-Gated Sodium Channels.Chembiochem : a European journal of chemical biology · 2023Article
- Development of Neuronal Guidance Fibers for Stimulating Electrodes: Basic Construction and Delivery of a Growth Factor.Frontiers in bioengineering and biotechnology · 2022Article
- Molecular-Scale Dynamics of Long Range Retrograde Brain-Derived Neurotrophic Factor Transport Shaped by Cellular Spatial Context.Frontiers in neuroscience · 2022Article
- Article
- Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB.Nature communications · 2016Article
- In vivo imaging of axonal transport in murine motor and sensory neurons.Journal of neuroscience methods · 2016Article
- Compartmentalized Platforms for Neuro-Pharmacological Research.Current neuropharmacology · 2016Review
- Activity-dependent BDNF release via endocytic pathways is regulated by synaptotagmin-6 and complexin.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Ligand-induced dynamics of neurotrophin receptors investigated by single-molecule imaging approaches.International journal of molecular sciences · 2015Review
- Article
- Nanoparticle-Based and Bioengineered Probes and Sensors to Detect Physiological and Pathological Biomarkers in Neural Cells.Frontiers in neuroscience · 2015Review
- "Extremely minimally invasive": recent advances in nanotechnology research and future applications in neurosurgery.Neurosurgical review · 2015Review
- Real-time imaging of axonal transport of quantum dot-labeled BDNF in primary neurons.Journal of visualized experiments : JoVE · 2014Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Brain derived neurotrophic factor (BDNF) plays a key role in the growth, development and maintenance of the central and peripheral nervous systems. Exogenous BDNF activates its membrane receptors at the axon terminal, and subsequently sends regulation signals to the cell body. To understand how a BDNF signal propagates in neurons, it is important to follow the trafficking of BDNF after it is internalized at the axon terminal. Here we labeled BDNF with bright, photostable quantum dots (QD-BDNF) and followed the axonal transport of QD-BDNF in real time in hippocampal neurons. We showed that QD-BDNF was able to bind BDNF receptors and activate downstream signaling pathways. When QD-BDNF was applied to the distal axons of hippocampal neurons, it was observed to be actively transported toward the cell body at an average speed of 1.11 ± 0.05 μm s(-1). A closer examination revealed that QD-BDNF was transported by both discrete endosomes and multivesicular body-like structures. Our results showed that QD-BDNF could be used to track the movement of exogenous BDNF in neurons over long distances and to study the signaling organelles that contain BDNF.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.