Evidence map›Paper›PMID 22772310›Full record

ArticleMycorrhiza2013

Quantification of extraradical mycelium of Tuber melanosporum in soils from truffle orchards in northern Spain.

Javier Parladé, Herminia De la Varga, Ana Ma De Miguel, Raimundo Sáez, Joan Pera

Abstract readEvaluation Study
PubMed Publisher
In one paragraph

Article in Mycorrhiza, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed.

  1. Rapid detection of cultivated truffle fungi (Applied and environmental microbiology · 2026
    Article
  2. Review
  3. Article
  4. Article
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  6. Article
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  8. Article
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  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Fungi as a Source of Food.Microbiology spectrum · 2017
    Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Javier ParladéIRTA, Sustainable Plant Protection, Centre de Cabrils, Ctra. Cabrils km. 2, 08348 Cabrils, Barcelona, Spain. xavier.parlade@irta.es
Herminia De la Varga
Ana Ma De Miguel
Raimundo Sáez
Joan Pera

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantification of extraradical mycelium of black truffle (Tuber melanosporum) has been carried out in a natural truffle ground and in seven truffle orchards (around 20 years old) established in Tierra Estella and Valdorba sites, within the natural distribution area of the black truffles in Navarre (northern Spain). Specific primers and a Taqman® probe were designed to perform real-time PCR with DNA extracted from soil samples. Amplification of T. melanosporum DNA was obtained from 131 out of the 160 soil samples. The detection limit of the technique was 1.48 μg mycelium/g of soil. The extraradical mycelium biomass detected in the soil from the natural truffle ground was significantly greater (up to ten times higher) than the mycelium biomass detected in any of the orchards. Soil from productive, nonirrigated orchards in the Tierra Estella site contained significantly more extraradical mycelium than the rest of orchards irrigated, productive of T. brumale, or nonproductive. The comparison of soil mycelium biomass in nonirrigated evergreen oak orchards in both sites showed significantly more mycelium biomass in the Tierra Estella site. This study is the first attempt to quantify extraradical mycelium of T. melanosporum in the soil using Taqman® probes. The obtained quantitative results are of special interest to evaluate the fungal response to cultural treatments and to monitor the dynamics of the extraradical mycelium of T. melanosporum in the soil.

Indexed as

Soil MicrobiologyAscomycotaColony Count, MicrobialDNA, FungalDNA PrimersMyceliumOligonucleotide ProbesReal-Time Polymerase Chain ReactionSensitivity and SpecificitySpainDNA, FungalDNA PrimersOligonucleotide Probes

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.