ArticleThe Journal of cell biology1990
A lamin-independent pathway for nuclear envelope assembly.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 121 papers.
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Who cites it
121 citing papers in PubMed.
- Mitotic dynamics of the nuclear lamina in the backstage of chromosome separation.Communications biology · 2025Review
- Tubular Lamin- and Emerin-Lined Nuclear Envelope Invaginations Isolate Chromatin Domains for Gene Expression Regulation.Sub-cellular biochemistry · 2025Review
- Nuclear growth and import can be uncoupled.Molecular biology of the cell · 2024Article
- Nucleus size and its effect on nucleosome stability in living cells.Biophysical journal · 2022Article
- Control of nuclear size by osmotic forces ineLife · 2022Article
- Size regulation of multiple organelles competing for a limiting subunit pool.PLoS computational biology · 2022Article
- Nuclear Lamins: Key Proteins for Embryonic Development.Biology · 2022Review
- Article
- Review
- VCP maintains nuclear size by regulating the DNA damage-associated MDC1-p53-autophagy axis in Drosophila.Nature communications · 2021Article
- Akt1-associated actomyosin remodelling is required for nuclear lamina dispersal and nuclear shrinkage in epidermal terminal differentiation.Cell death and differentiation · 2021Article
- Organelle size scaling over embryonic development.Wiley interdisciplinary reviews. Developmental biology · 2020Review
- Review
- The Perinuclear ER Scales Nuclear Size Independently of Cell Size in Early Embryos.Developmental cell · 2020Article
- Nucleoplasmin is a limiting component in the scaling of nuclear size with cytoplasmic volume.The Journal of cell biology · 2019Article
- The nucleoporin ELYS regulates nuclear size by controlling NPC number and nuclear import capacity.EMBO reports · 2019Article
- The Biology of the Nuclear Envelope and Its Implications in Cancer Biology.International journal of molecular sciences · 2019Review
- Nuclear import of Xenopus egg extract components into cultured cells for reprogramming purposes: a case study on goldfish fin cells.Scientific reports · 2019Article
- Colloid osmotic parameterization and measurement of subcellular crowding.Molecular biology of the cell · 2019Review
- Micronuclei Formation Is Prevented by Aurora B-Mediated Exclusion of HP1a from Late-Segregating Chromatin inGenetics · 2018Article
61 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The nuclear envelope is composed of membranes, nuclear pores, and a nuclear lamina. Using a cell-free nuclear assembly extract derived from Xenopus eggs, we have investigated how these three components interact during nuclear assembly. We find that the Xenopus embryonic lamin protein LIII cannot bind directly to chromatin or membranes when each is present alone, but is readily incorporated into nuclei when both of the components are present together in an assembly extract. We find that depleting lamin LIII from an extract does not prevent formation of an envelope consisting of membranes and nuclear pores. However, these lamin-depleted envelopes are extremely fragile and fail to grow beyond a limited extent. This suggests that lamin assembly is not required during the initial steps of nuclear envelope formation, but is required for later growth and for maintaining the structural integrity of the envelope. We also present results showing that lamins may only be incorporated into nuclei after DNA has been encapsulated within an envelope and nuclear transport has been activated. With respect to nuclear function, our results show that the presence of a nuclear lamina is required for DNA synthesis to occur within assembled nuclei.
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Registered trials
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