ArticleBiophysical journal2012
Characterization of cholesterol crystals in atherosclerotic plaques using stimulated Raman scattering and second-harmonic generation microscopy.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 153 citations in OpenAlex.
- AI-Assisted Coherent Raman Scattering Microscopy for Clinical Translation.Chemical & biomedical imaging · 2026Review
- Hepatic management of toxic sterols after acute deletion of Cyp51 from cholesterol synthesis.The Journal of biological chemistry · 2026Article
- Stimulated Raman Scattering Imaging Enabled Biomarker Discovery for Precision Medicine.Chemical & biomedical imaging · 2025Review
- Design Principles Of Inorganic-Protein Hybrid Materials for Biomedicine.Exploration (Beijing, China) · 2025Review
- Hyper spectral resolution stimulated Raman spectroscopy with amplified fs pulse bursts.Light, science & applications · 2024Article
- Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature.Exploration (Beijing, China) · 2023Review
- Raman Imaging Reveals Insights into Membrane Phase Biophysics in Cells.The journal of physical chemistry. B · 2023Review
- Stimulated Raman Scattering Imaging Sheds New Light on Lipid Droplet Biology.The journal of physical chemistry. B · 2023Article
- Simultaneous Dual-Band Hyperspectral Stimulated Raman Scattering Microscopy with Femtosecond Optical Parametric Oscillators.The journal of physical chemistry. B · 2023Article
- Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy.Photoacoustics · 2022Article
- Pathophysiology of Atherosclerosis.International journal of molecular sciences · 2022Review
- Multi-color stimulated Raman scattering with a frame-to-frame wavelength-tunable fiber-based light source.Biomedical optics express · 2021Article
- Coherent Raman scattering microscopy: capable solution in search of a larger audience.Journal of biomedical optics · 2021Article
- Localisation and regulation of cholesterol transporters in the human hair follicle: mapping changes across the hair cycle.Histochemistry and cell biology · 2021Article
- Optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoEScientific reports · 2021Article
- Article
- Label-free multiphoton microscopy as a tool to investigate alterations of cerebral aneurysms.Scientific reports · 2020Article
- Bile acid sequestration reverses liver injury and prevents progression of nonalcoholic steatohepatitis in Western diet-fed mice.The Journal of biological chemistry · 2020Article
- Linear and planar reflection artifacts on swept-source and spectral-domain optical coherence tomography due to hyperreflective crystalline deposits.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2020Article
- Cholesterol Crystals and NLRP3 Mediated Inflammation in the Uterine Wall Decidua in Normal and Preeclamptic Pregnancies.Frontiers in immunology · 2020Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Cholesterol crystals (ChCs) have been identified as a major factor of plaque vulnerability and as a potential biomarker for atherosclerosis. Yet, due to the technical challenge of selectively detecting cholesterol in its native tissue environment, the physiochemical role of ChCs in atherosclerotic progression remains largely unknown. In this work, we demonstrate the utility of hyperspectral stimulated Raman scattering (SRS) microscopy combined with second-harmonic generation (SHG) microscopy to selectively detect ChC. We show that despite the polarization sensitivity of the ChC Raman spectrum, cholesterol monohydrate crystals can be reliably discriminated from aliphatic lipids, from structural proteins of the tissue matrix and from other condensed structures, including cholesteryl esters. We also show that ChCs exhibit a nonvanishing SHG signal, corroborating the noncentrosymmetry of the crystal lattice composed of chiral cholesterol molecules. However, combined hyperspectral SRS and SHG imaging reveals that not all SHG-active structures with solidlike morphologies can be assigned to ChCs. This study exemplifies the merit of combining SRS and SHG microscopy for an enhanced label-free chemical analysis of crystallized structures in diseased tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.