ArticleBiophysical journal2012
Microscopic analysis of bacterial motility at high pressure.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Rapid changes in hydrostatic pressure as a probe for correlating function of purified proteins with their measured activity in living cells.Biophysical reviews · 2025Review
- Effect of shear rate on earlyBiofilm · 2024Article
- Novel Amiloride Derivatives That Inhibit Bacterial Motility across Multiple Strains and Stator Types.Journal of bacteriology · 2021Article
- Pressure-induced changes on the morphology and gene expression in mammalian cells.Biology open · 2021Article
- High pressure inhibits signaling protein binding to the flagellar motor and bacterial chemotaxis through enhanced hydration.Scientific reports · 2020Article
- Dissimilatory Sulfate Reduction Under High Pressure byFrontiers in microbiology · 2018Article
- Bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness.Scientific reports · 2016Article
- Sodium-driven energy conversion for flagellar rotation of the earliest divergent hyperthermophilic bacterium.Scientific reports · 2015Article
- A tale of two machines: a review of the BLAST meeting, Tucson, AZ, 20-24 January 2013.Molecular microbiology · 2014Review
- Single-molecule analysis of the rotation of F₁-ATPase under high hydrostatic pressure.Biophysical journal · 2013Article
- High hydrostatic pressure induces counterclockwise to clockwise reversals of the Escherichia coli flagellar motor.Journal of bacteriology · 2013Article
- Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.PloS one · 2013Article
- Bacterial motility measured by a miniature chamber for high-pressure microscopy.International journal of molecular sciences · 2012Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial flagellar motor is a molecular machine that converts an ion flux to the rotation of a helical flagellar filament. Counterclockwise rotation of the filaments allows them to join in a bundle and propel the cell forward. Loss of motility can be caused by environmental factors such as temperature, pH, and solvation. Hydrostatic pressure is also a physical inhibitor of bacterial motility, but the detailed mechanism of this inhibition is still unknown. Here, we developed a high-pressure microscope that enables us to acquire high-resolution microscopic images, regardless of applied pressures. We also characterized the pressure dependence of the motility of swimming Escherichia coli cells and the rotation of single flagellar motors. The fraction and speed of swimming cells decreased with increased pressure. At 80 MPa, all cells stopped swimming and simply diffused in solution. After the release of pressure, most cells immediately recovered their initial motility. Direct observation of the motility of single flagellar motors revealed that at 80 MPa, the motors generate torque that should be sufficient to join rotating filaments in a bundle. The discrepancy in the behavior of free swimming cells and individual motors could be due to the applied pressure inhibiting the formation of rotating filament bundles that can propel the cell body in an aqueous environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.