ArticleBiophysical journal2012
The probability of triggering calcium puffs is linearly related to the number of inositol trisphosphate receptors in a cluster.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Stochastic model of IP3-induced Ca2+ spiking of HEK293 cells.PLoS computational biology · 2025Article
- A CaJournal of mathematical biology · 2025Article
- Modeling IPBiophysical journal · 2023Article
- An integrate-and-fire approach to CaBiophysical journal · 2023Article
- Computational investigation of IPScientific reports · 2023Article
- Inositol 1,4,5-trisphosphate receptors in cardiomyocyte physiology and disease.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- KRAP is required for diffuse and punctate IPCell calcium · 2022Article
- Mechanisms and physiological implications of cooperative gating of clustered ion channels.Physiological reviews · 2022Review
- Termination of CaCell calcium · 2021Article
- IPProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Lessons from the Endoplasmic Reticulum CaCells · 2020Review
- Noise analysis of cytosolic calcium image data.Cell calcium · 2020Article
- Spatial-temporal patterning of CaSeminars in cell & developmental biology · 2019Review
- Simulation of calcium signaling in fine astrocytic processes: Effect of spatial properties on spontaneous activity.PLoS computational biology · 2019Article
- All three IPScience signaling · 2018Article
- CaNature communications · 2017Article
- Mapping Interpuff Interval Distribution to the Properties of Inositol Trisphosphate Receptors.Biophysical journal · 2017Article
- Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action.Science signaling · 2016Article
- A comparison of fluorescent Ca²⁺ indicators for imaging local Ca²⁺ signals in cultured cells.Cell calcium · 2015Article
- On the Adjacency Matrix of RyR2 Cluster Structures.PLoS computational biology · 2015Article
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3 authors.
Funding
Abstract
Puffs are local Ca(2+) signals that arise by Ca(2+) liberation from the endoplasmic reticulum through concerted opening of tightly clustered inositol trisphosphate receptor/channels (IP(3)R). They serve both local signaling functions and trigger global Ca(2+) waves. The numbers of functional IP(3)R within clusters differ appreciably between different puff sites, and we investigated how the probability of puff occurrence varies with cluster size. We imaged puffs in SH-SY5Y cells using total internal fluorescence microscopy, and estimated cluster sizes from the magnitude of the largest puff observed at each site relative to the signal from a single channel. We find that the initial triggering rate of puffs following photorelease of IP(3), and the average frequency of subsequent repetitive puffs, vary about linearly with cluster size. These data accord well with stochastic simulations in which opening of any individual IP(3)R channel within a cluster triggers a puff via Ca(2+)-induced Ca(2+) release. An important consequence is that the signaling power of a puff site (average amount of Ca(2+) released per puff × puff frequency) varies about the square of cluster size, implying that large clusters contribute disproportionately to cellular signaling and, because of their higher puff frequency, preferentially act as pacemakers to initiate Ca(2+) waves.
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