ArticleBiophysical journal2012
Shear-induced resistance to neutrophil activation via the formyl peptide receptor.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Neutrophils actively swell to potentiate rapid migration.bioRxiv : the preprint server for biology · 2024Article
- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- Supercharged eGFP-TRAIL Decorated NETs to Ensnare and Kill Disseminated Tumor Cells.Cellular and molecular bioengineering · 2020Article
- Formyl peptide receptor-1 activation exerts a critical role for the dynamic plasticity of arteries via actin polymerization.Pharmacological research · 2019Article
- Effects of Transient Exposure to High Shear on Neutrophil Rolling Behavior.Cellular and molecular bioengineering · 2018Article
- Extension of chemotactic pseudopods by nonadherent human neutrophils does not require or cause calcium bursts.Science signaling · 2018Article
- Mechanical deformation induces depolarization of neutrophils.Science advances · 2017Article
- Lamin A/C deficiency reduces circulating tumor cell resistance to fluid shear stress.American journal of physiology. Cell physiology · 2015Article
- Immobilized surfactant-nanotube complexes support selectin-mediated capture of viable circulating tumor cells in the absence of capture antibodies.Journal of biomedical materials research. Part A · 2015Article
- Surfactant functionalization induces robust, differential adhesion of tumor cells and blood cells to charged nanotube-coated biomaterials under flow.Biomaterials · 2015Article
- Leukocytes as carriers for targeted cancer drug delivery.Expert opinion on drug delivery · 2015Review
- Article
- Highly permeable silicon membranes for shear free chemotaxis and rapid cell labeling.Lab on a chip · 2014Article
- Fluid shear stress increases neutrophil activation via platelet-activating factor.Biophysical journal · 2014Article
- Physical biology in cancer. 3. The role of cell glycocalyx in vascular transport of circulating tumor cells.American journal of physiology. Cell physiology · 2014Review
- Stem cell enrichment with selectin receptors: mimicking the pH environment of trauma.Sensors (Basel, Switzerland) · 2013Article
- Shear Stress Enhances Chemokine Secretion fromCellular and molecular bioengineering · 2013Article
- Fluid Shear Stress Sensitizes Cancer Cells to Receptor-Mediated Apoptosis via Trimeric Death Receptors.New journal of physics · 2013Article
- Computational and experimental models of cancer cell response to fluid shear stress.Frontiers in oncology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The application of fluid shear stress on leukocytes is critical for physiological functions including initial adhesion to the endothelium, the formation of pseudopods, and migration into tissues. The formyl peptide receptor (FPR) on neutrophils, which binds to formyl-methionyl-leucyl-phenylalanine (fMLP) and plays a role in neutrophil chemotaxis, has been implicated as a fluid shear stress sensor that controls pseudopod formation. The role of shear forces on earlier indicators of neutrophil activation, such as L-selectin shedding and α(M)β(2) integrin activation, remains unclear. Here, human neutrophils exposed to uniform shear stress (0.1-4.0 dyn/cm(2)) in a cone-and-plate viscometer for 1-120 min showed a significant reduction in both α(M)β(2) integrin activation and L-selectin shedding after stimulation with 0.5 nM of fMLP. Neutrophil resistance to activation was directly linked to fluid shear stress, as the response increased in a shear stress force- and time-dependent manner. Significant shear-induced loss of FPR surface expression on neutrophils was observed, and high-resolution confocal microscopy revealed FPR internalized within neutrophils. These results suggest that physiological shear forces alter neutrophil activation via FPR by reducing L-selectin shedding and α(M)β(2) integrin activation in the presence of soluble ligand.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.