ArticleParasites & vectors2012
Functional characterization of an arrestin gene on insecticide resistance of Culex pipiens pallens.
Article in Parasites & vectors, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 26 citations in OpenAlex.
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- Signatures of soft selective sweeps predominate in the yellow fever mosquitobioRxiv : the preprint server for biology · 2025Article
- Genome wide population genetics and molecular surveillance of insecticide resistance in Anopheles stephensi mosquitoes from Awash Sebat Kilo in Ethiopia.Scientific reports · 2025Article
- Knockdown resistance (kdr)-associated organochlorine resistance in mosquito-borne diseases (Heliyon · 2025Article
- Unveiling the Role of Two Rhodopsin-like GPCR Genes in Insecticide-Resistant House Flies,International journal of molecular sciences · 2024Article
- Multiple-P450 Gene Co-Up-Regulation in the Development of Permethrin Resistance in the House Fly,International journal of molecular sciences · 2023Article
- G-Protein Coupled Receptors (GPCRs) in Insects-A Potential Target for New Insecticide Development.Molecules (Basel, Switzerland) · 2021Review
- G-Protein Coupled Receptors (GPCRs): Signaling Pathways, Characterization, and Functions in Insect Physiology and Toxicology.International journal of molecular sciences · 2021Review
- Ocular Albinism Type 1 Regulates Deltamethrin Tolerance inFrontiers in physiology · 2019Article
- CYP-mediated permethrin resistance in Aedes aegypti and evidence for trans-regulation.PLoS neglected tropical diseases · 2018Article
- NYD-OP7/PLC regulatory signaling pathway regulates deltamethrin resistance in Culex pipiens pallens (Diptera: Culicidae).Parasites & vectors · 2018Article
- Venom allergen 5 is Associated With Deltamethrin Resistance in Culex pipiens pallens (Diptera: Culicidae).Journal of medical entomology · 2015Article
- Identification of QTLs Conferring Resistance to Deltamethrin in Culex pipiens pallens.PloS one · 2015Article
- Transcriptome profiling of pyrethroid resistant and susceptible mosquitoes in the malaria vector, Anopheles sinensis.BMC genomics · 2014Article
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14 authors at 1 institution in 1 country.
Funding
Abstract
backgroundContinuous and excessive application of insecticides has resulted in the rapid development of insecticide resistance in several mosquito species, including Culex pipiens pallens. Previous studies in our laboratory found that arrestin gene expression was higher in the deltamethrin-resistant (DR) strain than in the deltamethrin-susceptible (DS) strain of Cx. pipiens pallens. Similarly, other studies reported that arrestin was highly expressed in permethrin-resistant Cx. quinquefasciatus and in dichlorodiphenyltrichloroethane (DDT)-resistant Drosophila melanogaster.
methodsFull-length cDNAs of an arrestin gene were cloned from Cx. pipiens pallens via polymerase chain reaction (PCR) and rapid amplification of cDNA end (RACE). The mRNA levels of the arrestin gene in the whole life cycle of DR and DS strains of Cx. pipiens pallens were investigated via quantitative real-time PCR. In addition, the relationship between arrestin and deltamethrin (DM) resistance were identified using genetic overexpression strategies and arrestin RNAi in mosquito cells. Cell viability was analyzed with cholecystokinin octapeptide after DM treatment. Moreover, the mRNA levels of cytochrome P450 6A1 (CYP6A1) and opsin in the transfected cells and controls were analyzed.
resultsComplete arrestin gene sequence was cloned and expressed throughout the life cycle of Cx. pipiens pallens. Moreover, arrestin was significantly upregulated in the DR strain, compared with that in the DS strain at the egg, pupae, and adult stages. Arrestin overexpression comparably increased the mosquito cell viability, whereas arrestin knockdown by siRNA decreased mosquito cell viability with deltamethrin (DM) treatment. Meanwhile, the mRNA levels of CYP6A1 and opsin were upregulated in mosquito cells transfected with arrestin and downregulated in mosquito cells with arrestin knockdown.
conclusionThis study presented the first evidence that arrestin might be associated with insecticide resistance in Cx. pipiens pallens.
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