ArticlePloS one2012
Real-time measurement of F-actin remodelling during exocytosis using Lifeact-EGFP transgenic animals.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Autophagy, Acute Pancreatitis and the Metamorphoses of a Trypsinogen-Activating Organelle.Cells · 2022Review
- LAP-like non-canonical autophagy and evolution of endocytic vacuoles in pancreatic acinar cells.Autophagy · 2020Article
- Actin and Myosin in Non-Neuronal Exocytosis.Cells · 2020Review
- A new bioluminescence-based tool for modulating target proteins in live cells.Scientific reports · 2019Article
- Intracellular rupture, exocytosis and actin interaction of endocytic vacuoles in pancreatic acinar cells: initiating events in acute pancreatitis.The Journal of physiology · 2018Article
- Parallel assembly of actin and tropomyosin, but not myosin II, duringJournal of cell science · 2018Article
- Modulation of paracrine signaling by CD9 positive small extracellular vesicles mediates cellular growth of androgen deprived prostate cancer.Oncotarget · 2017Article
- Article
- Small GTPases promote actin coat formation on microsporidian pathogens traversing the apical membrane of Caenorhabditis elegans intestinal cells.Cellular microbiology · 2016Article
- Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.Nature communications · 2015Article
- Imaging membrane remodeling during regulated exocytosis in live mice.Experimental cell research · 2015Review
- In vivo imaging of Nematostella vectensis embryogenesis and late development using fluorescent probes.BMC cell biology · 2014Article
- Ca²⁺-regulated secretory granule exocytosis in pancreatic and parotid acinar cells.Cell calcium · 2014Review
- The cortical acto-Myosin network: from diffusion barrier to functional gateway in the transport of neurosecretory vesicles to the plasma membrane.Frontiers in endocrinology · 2013Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
F-actin remodelling is essential for a wide variety of cell processes. It is important in exocytosis, where F-actin coats fusing exocytic granules. The purpose of these F-actin coats is unknown. They may be important in stabilizing the fused granules, they may play a contractile role and promote expulsion of granule content and finally may be important in endocytosis. To elucidate these functions of F-actin remodelling requires a reliable method to visualize F-actin dynamics in living cells. The recent development of Lifeact-EGFP transgenic animals offers such an opportunity. Here, we studied the characteristics of exocytosis in pancreatic acinar cells obtained from the Lifeact-EGFP transgenic mice. We show that the time-course of agonist-evoked exocytic events and the kinetics of each single exocytic event are the same for wild type and Lifeact-EGFP transgenic animals. We conclude that Lifeact-EGFP animals are a good model to study of exocytosis and reveal that F-actin coating is dependent on the de novo synthesis of F-actin and that development of actin polymerization occurs simultaneously in all regions of the granule. Our insights using the Lifeact-EGFP mice demonstrate that F-actin coating occurs after granule fusion and is a granule-wide event.
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