ArticlePloS one2012
Interplay of Nkx3.2, Sox9 and Pax3 regulates chondrogenic differentiation of muscle progenitor cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Engineered muscle drives bone repair and functional healing after composite musculoskeletal injury.Biomaterials · 2026Article
- PAX3: A Driver of Normal Development and Disease.Biomolecules · 2026Review
- Article
- Inhibition of miR-101-3p prevents human aortic valve interstitial cell calcification through regulation of CDH11/SOX9 expression.Molecular medicine (Cambridge, Mass.) · 2023Article
- Interplay between integrins and cadherins to control bone differentiation upon BMP-2 stimulation.Frontiers in cell and developmental biology · 2022Article
- Direct contribution of skeletal muscle mesenchymal progenitors to bone repair.Nature communications · 2021Article
- Identification of markers associated with estimated breeding value and horn colour in Hungarian Grey cattle.Animal bioscience · 2021Article
- Temporal TGF-β Supergene Family Signalling Cues Modulating Tissue Morphogenesis: Chondrogenesis within a Muscle Tissue Model?International journal of molecular sciences · 2020Article
- miRNA-101 promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells.Experimental and therapeutic medicine · 2019Article
- Identification of satellite cells from anole lizard skeletal muscle and demonstration of expanded musculoskeletal potential.Developmental biology · 2018Article
- Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.Arthritis research & therapy · 2017Article
- Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation.European journal of histochemistry : EJH · 2017Article
- Harnessing Endogenous Cellular Mechanisms for Bone Repair.Frontiers in bioengineering and biotechnology · 2017Review
- The superior regenerative potential of muscle-derived stem cells for articular cartilage repair is attributed to high cell survival and chondrogenic potential.Molecular therapy. Methods & clinical development · 2016Article
- VAV3 Overexpressed in Cancer Stem Cells Is a Poor Prognostic Indicator in Ovarian Cancer Patients.Stem cells and development · 2015Article
- Muscle-bone interactions during fracture healing.Journal of musculoskeletal & neuronal interactions · 2015Review
- Article
- The role of Nkx3.2 in chondrogenesis.Frontiers in biology · 2014Article
- Molecular mechanisms of heterotopic ossification.The Journal of hand surgery · 2014Review
- Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Muscle satellite cells make up a stem cell population that is capable of differentiating into myocytes and contributing to muscle regeneration upon injury. In this work we investigate the mechanism by which these muscle progenitor cells adopt an alternative cell fate, the cartilage fate. We show that chick muscle satellite cells that normally would undergo myogenesis can be converted to express cartilage matrix proteins in vitro when cultured in chondrogenic medium containing TGFß3 or BMP2. In the meantime, the myogenic program is repressed, suggesting that muscle satellite cells have undergone chondrogenic differentiation. Furthermore, ectopic expression of the myogenic factor Pax3 prevents chondrogenesis in these cells, while chondrogenic factors Nkx3.2 and Sox9 act downstream of TGFß or BMP2 to promote this cell fate transition. We found that Nkx3.2 and Sox9 repress the activity of the Pax3 promoter and that Nkx3.2 acts as a transcriptional repressor in this process. Importantly, a reverse function mutant of Nkx3.2 blocks the ability of Sox9 to both inhibit myogenesis and induce chondrogenesis, suggesting that Nkx3.2 is required for Sox9 to promote chondrogenic differentiation in satellite cells. Finally, we found that in an in vivo mouse model of fracture healing where muscle progenitor cells were lineage-traced, Nkx3.2 and Sox9 are significantly upregulated while Pax3 is significantly downregulated in the muscle progenitor cells that give rise to chondrocytes during fracture repair. Thus our in vitro and in vivo analyses suggest that the balance of Pax3, Nkx3.2 and Sox9 may act as a molecular switch during the chondrogenic differentiation of muscle progenitor cells, which may be important for fracture healing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.