ArticlePloS one2012
Functional analysis of conserved motifs in the mechanosensitive channel homolog MscS-Like2 from Arabidopsis thaliana.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 35 citations in OpenAlex.
- Review
- Origin, evolution and diversification of plant mechanosensitive channel of small conductance-like (MSL) proteins.BMC plant biology · 2023Article
- Structural insights into the Venus flytrap mechanosensitive ion channel Flycatcher1.Nature communications · 2022Article
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- With an Ear Up against the Wall: An Update on Mechanoperception inPlants (Basel, Switzerland) · 2021Review
- Plant cell wall integrity maintenance in model plants and crop species-relevant cell wall components and underlying guiding principles.Cellular and molecular life sciences : CMLS · 2020Review
- Plastid osmotic stress influences cell differentiation at the plant shoot apex.Development (Cambridge, England) · 2016Article
- Nod Factor Effects on Root Hair-Specific Transcriptome of Medicago truncatula: Focus on Plasma Membrane Transport Systems and Reactive Oxygen Species Networks.Frontiers in plant science · 2016Article
- The Arabidopsis synaptotagmin1 is enriched in endoplasmic reticulum-plasma membrane contact sites and confers cellular resistance to mechanical stresses.Plant physiology · 2015Article
- The evolutionary 'tinkering' of MscS-like channels: generation of structural and functional diversity.Pflugers Archiv : European journal of physiology · 2015Review
- RNA Sequencing Analysis of the msl2msl3, crl, and ggps1 Mutants Indicates that Diverse Sources of Plastid Dysfunction Do Not Alter Leaf Morphology Through a Common Signaling Pathway.Frontiers in plant science · 2015Article
- United in diversity: mechanosensitive ion channels in plants.Annual review of plant biology · 2015Review
- Arabidopsis MSL10 has a regulated cell death signaling activity that is separable from its mechanosensitive ion channel activity.The Plant cell · 2014Article
- Plastid osmotic stress activates cellular stress responses in Arabidopsis.Plant physiology · 2014Article
- Structural characterization of the mechanosensitive channel candidate MCA2 from Arabidopsis thaliana.PloS one · 2014Article
- A force of nature: molecular mechanisms of mechanoperception in plants.Journal of experimental botany · 2013Review
- MscS-like mechanosensitive channels in plants and microbes.Biochemistry · 2013Review
- MscS inactivation: an exception rather than the rule. An extremophilic MscS reveals diversity within the family.Biophysical journal · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Mechanosensitive channel of Small conductance (MscS) of Escherichia coli has become an excellent model system for the structural, biophysical, and functional study of mechanosensitive ion channels. MscS, a complex channel with multiple states, contributes to protection against lysis upon osmotic downshock. MscS homologs are widely and abundantly dispersed among the bacterial and plant lineages, but are not found in animals. Investigation into the eukaryotic branch of the MscS family is in the beginning stages, and it remains unclear how much MscS homologs from eukaryotes resemble E. coli MscS with respect to structure, function, and regulation. Here we test the effect of mutating three conserved motifs on the function of MscS-Like (MSL)2, a MscS homolog localized to the plastids of Arabidopsis thaliana. We show that 1) a motif at the top of the cytoplasmic domain, referred to here as the PN(X)(9)N motif, is essential for MSL2 function and for its proper intraplastidic localization; 2) substituting polar residues for two large hydrophobic residues located in the predicted pore-lining transmembrane helix of MSL2 produces a likely gain-of-function allele, as previously shown for MscS; and 3) mis-expression of this allele causes severe defects in leaf growth, loss of chloroplast integrity, and abnormal starch accumulation. Thus, two of the three conserved motifs we analyzed are critical for MSL2 function, consistent with the conservation of structure and function between MscS family members in bacteria and plants. These results underscore the importance of plastidic mechanosensitive channels in the maintenance of normal plastid and leaf morphology.
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