ArticlePloS one2012
FLP recombinase-mediated site-specific recombination in silkworm, Bombyx mori.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 34 citations in OpenAlex.
- FLP/FRT-Mediated Recombination for Resistance Marker Recycling.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Very Early Corona Treatment-Mediated Artificial Incubation of Silkworm Eggs and Germline Transformation of Diapause Silkworm Strains.Frontiers in bioengineering and biotechnology · 2022Article
- Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii.BMC genetics · 2020Article
- Inducible Gene Switches with Memory in Human T Cells for Cellular Immunotherapy.ACS synthetic biology · 2019Article
- Highly efficient and inducible DNA excision in transgenic silkworms using the FLP/FRT site-specific recombination system.Transgenic research · 2016Article
- Genetically engineered mucin mouse models for inflammation and cancer.Cancer metastasis reviews · 2015Review
- Advanced technologies for genetically manipulating the silkworm Bombyx mori, a model Lepidopteran insect.Proceedings. Biological sciences · 2015Review
- An efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori.Scientific reports · 2015Article
- Site-specific cassette exchange systems in the Aedes aegypti mosquito and the Plutella xylostella moth.PloS one · 2015Article
- Construction of transformed, cultured silkworm cells and transgenic silkworm using the site-specific integrase system from phage φC31.Molecular biology reports · 2014Article
- A quicker degradation rate is yielded by a novel kind of transgenic silk fibroin consisting of shortened silk fibroin heavy chains fused with matrix metalloproteinase cleavage sites.Journal of materials science. Materials in medicine · 2014Article
- Real-time single-molecule tethered particle motion analysis reveals mechanistic similarities and contrasts of Flp site-specific recombinase with Cre and λ Int.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A comprehensive understanding of gene function and the production of site-specific genetically modified mutants are two major goals of genetic engineering in the post-genomic era. Although site-specific recombination systems have been powerful tools for genome manipulation of many organisms, they have not yet been established for use in the manipulation of the silkworm Bombyx mori genome. In this study, we achieved site-specific excision of a target gene at predefined chromosomal sites in the silkworm using a FLP/FRT site-specific recombination system. We first constructed two stable transgenic target silkworm strains that both contain a single copy of the transgene construct comprising a target gene expression cassette flanked by FRT sites. Using pre-blastoderm microinjection of a FLP recombinase helper expression vector, 32 G3 site-specific recombinant transgenic individuals were isolated from five of 143 broods. The average frequency of FLP recombinase-mediated site-specific excision in the two target strains genome was approximately 3.5%. This study shows that it is feasible to achieve site-specific recombination in silkworms using the FLP/FRT system. We conclude that the FLP/FRT system is a useful tool for genome manipulation in the silkworm. Furthermore, this is the first reported use of the FLP/FRT system for the genetic manipulation of a lepidopteran genome and thus provides a useful reference for the establishment of genome manipulation technologies in other lepidopteran species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.